Monitoring nosocomial infection caused by Candida in west region of the State of São Paulo: phenotypic and genotypic characterizations and correlation with the risk factors in patients with cancer

• Grant number: 14/16507-0
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2015
• End date: June 30, 2017
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luciana da Silva Ruiz Menezes
• Grantee: Luciana da Silva Ruiz Menezes
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Infections caused by Candida species are a major public health problem in tertiary hospitals all over the world, especially in patients with cancer, in which is associated with high mortality. Thus, for the purpose of helping to change this framework, this study will have the following objectives: 1) determine the frequency of yeast species isolated from clinical specimens in specific tertiary public hospital in the care of cancer patients in São Paulo - SP, 2) verify by molecular methods, the presence of C. dubliniensis among the phenotypes of C. albicans, 3) verify, by molecular methods, the prevalence of C. parapsilosis sensu stricto, C. orthopsilosis, C. metapsilosis, C. glabrata sensu stricto, C. bracarensis and C. nivariensis; 4) determine and compare the virulence factors: production of proteinase, phospholipase and hemolysin enzymes; 5) determine and compare the ability of samples to produce biofilm, 6) evaluate the sensitivity patterns of the isolates front antifungals used in the clinic for through the E-test method; 7) study and compare the genotypic profile of isolates of C. albicans, C. parapsilosis and C. glabrata by analysis of microsatellite markers, also elucidating the occurrence of a possible hospital outbreak; 8) correlate the identified species with data for antifungal susceptibility and risk factors of patients in order to organize an epidemiological protocol. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARNONI, MARIANA VOLPE; PAULA, CLAUDETE RODRIGUES; AULER, MARCOS ERENO; NAOZUKA SIMOES, CIRILO CESAR; NAKANO, SHIRLEY; SZESZS, MARIA WALDEREZ; CARVALHO MELHEM, MARCIA DE SOUZA; RICHINI PEREIRA, VIRGINIA BODELAO; GARCES, HANS GARCIA; BAGAGLI, EDUARDO; et al. Infections Caused by Fusarium Species in Pediatric Cancer Patients and Review of Published Literature. Mycopathologia, v. 183, n. 6, p. 941-949, DEC 2018. (14/16507-0)