Phenotypic and genotypic characterization of Candida spp strains of candidemia in universitary hospital

• Grant number: 14/10628-0
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2014 - May 31, 2017
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Márcia Eliana da Silva Ferreira
• Grantee: Márcia Eliana da Silva Ferreira
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Roberto Martinez

Abstract

Since the 80s, the number of patients affected by invasive infections caused by Candida species has been increasing due the increase of immunocompromised patients, invasive procedures, and use of broad-spectrum antibiotics. These yeasts, which are part of the human microbiota, take advantage of imbalances on the host to cause infections, which can be superficial or invasive. The invasive infections, known as candidemia, can present a mortality rate up to 50% and they are mainly caused by Candida albicans, C. parapsilosis, C. glabrata and C. tropicalis. Their prevalence varies between countries and each species has special characteristics, which makes necessary their identification. In addition to the identification, characterization of isolates in epidemiological studies is relevant and can be done using the antifungal susceptibility tests and phenotypic and genotypic tests. In this context, this project purposes to characterize, phenotypically and genotypically, isolates from candidemia episodes occurred on clinics hospital of FMRP, as well as analyze their susceptibility to the antifungal agents used in the treatment of candidemia. For this, tests will be done to detect the presence of proteinase, phospholipase and hemolysin; biofilm formation ability; MLST, PFGE and MLP genotyping; and disk diffusion susceptibility method and broth microdilution method according to CLSI guidelines. It is expected; therefore, contribute to this disease epidemiology on the hospital and in Brazil. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANELA, HELIARA MARIA SPINA; CARDOSO, BARBARA; FRAZAO, MILIANE RODRIGUES; FALCAO, JULIANA PFRIMER; VITALI, LUCIA HELENA; MARTINEZ, ROBERTO; DA SILVA FERREIRA, MARCIA ELIANA. Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital. Brazilian Journal of Microbiology, v. 52, n. 2, FEB 2021. (14/10628-0, 13/23485-0)

SPINA CANELA, HELIARA MARIA; CARDOSO, BARBARA; VITALI, LUCIA HELENA; COELHO, HARNOLDO COLARES; MARTINEZ, ROBERTO; FERREIRA, MARCIA ELIANA DA SILVA. Prevalence, virulence factors and antifungal susceptibility of Candida spp. isolated from bloodstream infections in a tertiary care hospital in Brazil. MYCOSES, v. 61, n. 1, p. 11-21, JAN 2018. (13/23485-0, 14/10628-0)

BRANCINI, GUILHERME T. P.; BACHMANN, LUCIANO; FERREIRA, MARCIA ELIANA DA S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L.. Exposing Metarhizium acridum mycelium to visible light up-regulates a photolyase gene and increases photoreactivating ability. Journal of Invertebrate Pathology, v. 152, p. 35-37, FEB 2018. (15/24305-0, 14/10628-0, 16/11386-5, 13/50518-6, 10/06374-1)