Genotyping by microsatellite analysis of Candida parapsilosis (sensu stricto) isolates obtained from cases of Invasive Candidiasis

• Grant number: 19/17851-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): November 01, 2019
• Effective date (End): March 31, 2021
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Danilo Yamamoto Thomaz
• Grantee: Gabrielle Oliveira Marques Haddad Carvalho
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Candida spp. is responsible for over 80% of cases of nosocomial fungal infection, representing an important cause of morbidity and mortality. Among the Candida species, Candida parapsilosis has been the most prevalent in neonatal intensive care units. The ability of this pathogen to form biofilm and colonize medical devices and hands of health professionals favors its horizontal transmission and the occurrence of hospital outbreaks. Several studies have revealed the genetic similarity between the clinical isolates and isolates obtained from hands of these professionals. Therefore, investigations on the molecular epidemiology of C. parapsilosis (sensu stricto), predominant species of C. parapsilosis complex, have been performed. However, low discriminatory methodologies such as PFGE and RAPD have been used for molecular typing. Thus, the employment of more discriminating genotyping methods is highly relevant for elucidating the dispersion and persistence of this yeast in the hospital environment. The present study aims to determine the genotypes of C. parapsilosis (sensu stricto) clinical isolates in a large tertiary Brazilian hospital by microsatellite analysis, which is one of the main molecular typing tools used in investigations of hospital outbreaks of candidemia.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THOMAZ, DANILO Y.; DEL NEGRO, GILDA M. B.; RIBEIRO, LEIDIANE B.; DA SILVA, MIRIAN; CARVALHO, GABRIELLE O. M. H.; CAMARGO, CARLOS H.; DE ALMEIDA, JOAO N.; MOTTA, ADRIANA L.; SICILIANO, RINALDO F.; SEJAS, ODELI N. E.; et al. A Brazilian Inter-Hospital Candidemia Outbreak Caused by Fluconazole-Resistant Candida parapsilosis in the COVID-19 Era. JOURNAL OF FUNGI, v. 8, n. 2, p. 8-pg., 2022-02-01. (18/19347-4, 18/15491-3, 21/00871-8, 19/17851-0)

THOMAZ, DANILO Y.; DE ALMEIDA, JOAO N.; SEJAS, ODELI N. E.; DEL NEGRO, GILDA M. B.; CARVALHO, GABRIELLE O. M. H.; GIMENES, VIVIANE M. F.; DE SOUZA, MARIA EMILIA B.; ARASTEHFAR, AMIR; CAMARGO, CARLOS H.; MOTTA, ADRIANA L.; et al. Environmental Clonal Spread of Azole-Resistant Candida parapsilosis with Erg11-Y132F Mutation Causing a Large Candidemia Outbreak in a Brazilian Cancer Referral Center. JOURNAL OF FUNGI, v. 7, n. 4, APR 2021. (19/17851-0, 17/00231-3, 18/19347-4, 16/08730-6)