CD25 T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses

• Grant number: 12/03859-0
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: April 01, 2012
• End date: September 30, 2012
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Ana Paula Campanelli
• Grantee: Ana Paula Campanelli
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) constitutes a microenvironment that could modulate the antitumor immune response. Also, tumor-infiltrating lymphocytes are believed to play complex regulatory roles in antitumor immunity against SCC. The presence of regulatory T cells (Tregs) has been associated with the suppression of tumor-reactive T cells. However, the underlying mechanism for this T cell dysfunction is not clear. We used a multistage model of SCC to examine the role of Treg cells during tumor development.7,12-dimethylbenz[a]-anthracene/phorbol 12-myristate 13-acetate treatment and systemic depletion of Treg cells using an anti-CD25 monoclonal antibody (PC61) resulted in a decrease in the number and incidence of papilloma. Furthermore, CD25 depletionincreased the proportion of CD81 and CD41 T cells that were isolated from tumor lesions. The levels of interleukin (IL)-1b, IL-10, IL-12, IL-13, interferon-g, transforming growth factor b and tumor necrosis factor-a, but not IL-17, were increased in the tumor microenvironment after Treg depletion. Therefore, our results indicated involvement of CD251 T cells in SCC developmentand in the suppression of the inflammatory immune response. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS, RODRIGO NALIO; OLIVEIRA, CARINE ERVOLINO; GASPAROTO, THAIS HELENA; DE SOUZA MALASPINA, TATIANA SALLES; BELAI, EDUARDO BERTOLI; CAVASSANI, KAREN ANGELICA; GARLET, GUSTAVO POMPERMAIER; DA SILVA, JOAO SANTANA; CAMPANELLI, ANA PAULA. CD25(+) T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses. Carcinogenesis, v. 33, n. 4, p. 902-909, APR 2012. (08/10999-7, 09/14127-7, 09/03471-9, 12/03859-0)