Frequency evaluation of cytogenetic and molecular alterations in AML at diagnosis and sequentially in the remission

• Grant number: 11/51751-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2012
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Maria de Lourdes Lopes Ferrari Chauffaille
• Grantee: Maria de Lourdes Lopes Ferrari Chauffaille
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The Acute Myeloid Leukemias (AML) are clonal neoplasic diseases genetically heterogeneous, characterized by somatic alterations in hematopoietic stem cells that modified the normal mechanisms of self-renew, proliferation and differentiation of these cells. The distinct clinical characteristics in each disease require differential diagnosis to determine the disease classification and the appropriate therapy. Recently a large number of genetic mutations and deregulated expressions of these genes have been identified, emerging as new diagnostic and prognostic factors and targets for new therapies, such as: NPM1, FLT3, WT1, CEBPA, K-Ras, N-Ras, MLL, RUNX1 e c-KIT. However, there are still a few studies among us that assess the range of cytogenetic and molecular alterations at diagnosis in patients with AML and no one covering all the listed genes. According to the studies so far conducted at Unifesp, as well as others conducted in collaboration, the complete remission rate and overall survival is shorter than the expected. So the best knowledge of the disease's genetic is critically important to contribute to the understanding of their biology and program improvements. Armed with this knowledge would be interesting evaluate, in hematologic remission, the level of residual disease as well as subsequently at the end of intensification. It would also be useful to compare the observed alterations with the clinical characteristics in the presentation and the outcome of evolution. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THIAGO RODRIGO DE NORONHA; SANDRA SERSON ROHR; MARIA DE LOURDES LOPES FERRARI CHAUFFAILLE. Identifying the similarities and differences between single nucleotide polymorphism array (SNPa) analysis and karyotyping in acute myeloid leukemia and myelodysplastic syndromes. Revista Brasileira de Hematologia e Hemoterapia, v. 37, n. 1, p. 48-54, 2015-02-00. (11/51751-0)