Study of cell activation and cytokines in cutaneous repair in diabetic rats treated with low level laser therapy

• Grant number: 12/01944-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: May 31, 2014
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Cristiane Miranda Franca
• Grantee: Cristiane Miranda Franca
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

The healing process of diabetic patients is often inefficient leading to chronic ulcers. Laser therapy is a promising tool to assist the closure of these wounds, but lack of standardization of dosimetry and understanding of their cellular and molecular mechanisms of action limit their use. This study aims to evaluate cell activation and cytokines in wound repair in diabetic animals which underwent laser therapy comparing two systems of delivery of laser light. Will be used 54 male Wistar rats (Rattus norvegicus albinus) with diabetes induced by intraperitoneal injection of streptozotocin (50mg/kg). The ulcer will be made using a 8 mm-punch in the back of the animal after trichotomy. Experimental groups: (1) control group (CG) - the sore back will not be subjected to treatment, (2) single dose (SDG) - dorsal ulcer will receive laser therapy two hours after the wound, (3) fractionated dose (FDG) - the ulcer will receive laser therapy on days 0, 3, 8, 10. Laser parameters for application in SGD will be: » = (660 ± 2) nm, I = 6mW/cm2, D= 4 J/cm2, t = 668 s; and for FDG group will be four doses of » = (660 ± 2) nm, I = 6mW/cm2, D= 1 J/cm2, t = 167 s totalizing 4 J/cm2. At days 0, 3, 8, 10, 14 e 21 the animals will be euthanized and the ulcers will be removed, routinely processed for hematoxylin & eosin stain and for immunohistochemistry to the following antigens: CD3, CD45RO, elastase, CD68, CD31, IL-1, IL-8, MCP-1 and VEGF. It will be assessed the presence and type of the inflammatory infiltrate, presence of granulation tissue, number of lymphocyte T, activated T lymphocytes, neutrophils, macrophages, angiogenic cells, as well as the production of cytokines above mentioned. The data will be assessed for normality and subjected to appropriate statistical analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, CRISTIANO DE LOURA; TEIXEIRA SILVA, DANIELA DE FATIMA; DE SOUZA, AMANDA PIRES; JACINTO, MARCOS VINICIUS; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI; SANTOS FERNANDES, KRISTIANNE PORTA; FRANCA, CRISTIANE MIRANDA. Effect of Laser Therapy on Immune Cells Infiltrate After Excisional Wounds in Diabetic Rats. Lasers in Surgery and Medicine, v. 48, n. 1, p. 45-51, JAN 2016. (12/01944-0)

SANTANA, CRISTIANO DE LOURA; TEIXEIRA SILVA, DANIELA DE FATIMA; DEANA, ALESSANDRO MELO; PRATES, RENATO ARAUJO; SOUZA, AMANDA PIRES; GOMES, MARIANA TEIXEIRA; DE AZEVEDO SAMPAIO, BRUNNA PILEGGI; SHIBUYA, JOSIANE FERRARETTO; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI; et al. Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds. PLoS One, v. 10, n. 4, APR 24 2015. (12/03334-4, 12/02801-8, 12/01944-0)

DEANA, ALESSANDRO MELO; COSTA DE JESUS, SERGIO HENRIQUE; AZEVEDO SAMPAIO, BRUNNA PILEGGI; OLIVEIRA, MARCELO TAVARES; TEIXEIRA SILVA, DANIELA FATIMA; FRANCA, CRISTIANE MIRANDA. Fully automated algorithm for wound surface area assessment. WOUND REPAIR AND REGENERATION, v. 21, n. 5, p. 755-761, SEP 2013. (12/02801-8, 12/01944-0)