Histological and histomorphometrical evaluation of the bone repair after application of natural latex and different bone grafts in skulls of Wistar rats

• Grant number: 12/03310-8
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2012
• End date: June 30, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: João Paulo Mardegan Issa
• Grantee: João Paulo Mardegan Issa
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Edilson Ervolino ; Joaquim Coutinho Netto ; Leda Maria Pescinini Salzedas

Abstract

The protein extracted from rubber trees (Hevea brasiliensis) has interesting properties with respect to the cicatrization of bone and soft tissues, being biocompatible and with high angiogenic potential. This study aims to evaluate the bone healing process in bone defects in rat calvaria using different types of bone grafts and P-1 protein extracted from Hevea brasiliensis (natural latex) and the association between these grafts and this protein, using histological, stereological, radiographical, immunohistochemical, biochemical and statistical methods. It will be used 112 male Wistar rats (250g) divided into 8 groups, each one with 4 and 6 weeks until the sacrifice. The groups are the following: 1) particulate autologous bone graft, 2) particulate homologous bone graft, 3) heterologous bone graft (lyophilized bovine bone), 4) particulate autologous bone graft + 5µg P-1, 5) homologous bone graft + 5µg P-1, 6) heterologous bone graft (lyophilized bovine bone) + 5µg P-1, 7) 5µg P-1, 8) critical bone defect only. The results for bone formation will be evaluated by the presented methods, and the resulting data will be subjected to statistical examination. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS KOTAKE, BRUNA GABRIELA; GONZAGA, MILIANE GONCALVES; COUTINHO-NETTO, JOAQUIM; ERVOLINO, EDILSON; DE FIGUEIREDO, FELLIPE A. T.; MARDEGAN ISSA, JOAO PAULO. Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1. Biomedical Materials, v. 13, n. 2, MAR 2018. (12/03310-8)