Electrophoresis of serum proteins in dogs with lymphoma of different phenotypes

• Grant number: 12/02613-7
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: November 30, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Áureo Evangelista Santana
• Grantee: Áureo Evangelista Santana
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The lymphoma is the tumor of hemolymphopoietic tissue more common in dogs and one of the most frequent malignant tumors in this species. An important supplement in diagnosis is to research immunophenotypic of the cell subpopulation involved and characterization of tumor of the substage established by the World Health Organization (WHO). This assay was conceived with the aim of indentifying lymphocytes subpopulations, were most commonly involved in the origin in the lymphoma, through the immunophenotyping of lymphocytes presents in the peripheral blood. Moreover, it is intend to immunocytochemistry analysis of lymph nodes, as well as electrophoresis of the serum proteins, and correlate the immunocytochemistry findings, immunophenotypic, hematologicals, proteics and clinical of the patient, at diagnosis of lymphoma. For this, will be evaluate 30 dogs, male or female, being 10 healthy and 20 with multicentric lymphoma, attending in the Veterinary Hospital "Governador Laudo Natel" da FCAV/UNESP, Jaboticabal, SP. The animals will be distribute in three experimental groups, a control group and in two groups of dogs with lymphoma. The control group (GC) will be consist of 10 healthy dogs, the group lymphoma B (GLB) will be formed with 10 dogs with lymphoma B and the group lymphoma T (GLT) will be composed with 10 dogs with lymphoma T. Therefore, want in this assay, from the electrophoresis profile of the serum proteins and the immunophenotypic characterization of the dogs with lymphoma, contribute to more accurate diagnosis and early lymphomatous state, as well as for the safer prognosis of this disease. (AU)