| Grant number: | 11/18506-2 |
| Support Opportunities: | Regular Research Grants |
| Start date: | July 01, 2012 |
| End date: | June 30, 2014 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal Investigator: | Marion Burkhardt de Koivisto |
| Grantee: | Marion Burkhardt de Koivisto |
| Host Institution: | Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil |
Abstract
The aim of this work is to investigate the rate of apoptosis in the ovary of female cats and its relationship with age. The female cats (n=18) will be classified into Group 1 (young up to one year), Group 2 (one to six years) and Group 3 (older than six years ). After ovariosalpingohysterectomy (OSH), the ovaries will be sectioned in order to obtain two equal halves, one being intended for evaluation of the ovarian tissue and the other investigation of apoptosis.. Apoptosis of the ovarian tissue will be detected by the TUNEL method. The maximum and minimum diameter (m), area (mm) and perimeter (mM) of the follicle and its oocyte of primordial follicles, primary unilaminar, multilaminar primary or preantral, secondary or antral and preovulatory follicles or Graafian will be measured. The relationship between the oocyte and follicle growth will be analyzed. Results will be submitted to analysis of variance (ANOVA) and means compared by Tukey test. Statistical analysis will be performed with the computer program SAS (Statistical Analysis System) version 9.2. (AU)
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