Study of micro-exon genes (MEGs) from the human parasite Schistosoma mansoni and its protein products

• Grant number: 12/09186-7
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Ricardo de Marco
• Grantee: Ricardo de Marco
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated researchers: Daniele Santini Jacinto

Abstract

This project aims to study micro-exon genes (MEGs) and its protein products that are located at the host parasite interface. We wil perform the heterologous expression of the main isoforms of PCMs (protein coded by micro-exon) to characterize their biophysical properties and to perform assays aiming to understand the role of these proteins. Double-hybrid assays will be performed to search for possible interaction between PCMs and proteins from human leukocytes. In addition, Bioinformatic analysis will compare the structure of MEGs in the three organisms from the Schistosoma genera that present a publicly avaliable genome sequence, the S. mansoni, S. japonicum and S. Haematobium, aiming to understand the evolution of this class of gene. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, p. 8-pg., 2017-01-01. (12/07288-7, 13/20715-4, 14/09361-9, 12/09186-7, 10/20290-5)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, A, p. 3490-3497, JAN 2017. (12/07288-7, 13/20715-4, 14/09361-9, 10/20290-5, 12/09186-7)