Valentine Anthony Lance | San Diego State University - United States

• Grant number: 05/01024-4
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: September 01, 2005
• End date: January 31, 2006
• Field of knowledge: Biological Sciences - Ecology - Applied Ecology
• Principal Investigator: Luciano Martins Verdade
• Grantee: Luciano Martins Verdade
• Visiting researcher: Valentine Anthony Lance
• Visiting researcher institution: San Diego State University, United States
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Phrynops geoffroanus will be captured monthly at Piracicamirim and Piracicaba rivers during the whole visit period with nylon fishing nets.Blood samples (5 ml) will collected from at least five adult male and five adult female turtles (up to ten samples from each sex will be collected if available) in every month of the year for one year. The blood will be held on ice until red cells are separated by centrifugation (an aliquot of whole blood, 100 ml will be set aside for any potential genetic analysis). The plasma will be divided into two separate tubes and frozen until ready for assay. The following steroids (testosterone, estradiol, progesterone, and corticostereone) will be measured in the plasma samples using radioimmunoassay (RIA) as described in the references below, or using the non-radioactive enzyme-linked immunoassay (ELISA), if available. If time permits, plasma calcium and magnesium will be assayed using atomic absorption spectrophotometry (AA), and analysis of plasma lipids using standard clinical techniques will be undertaken. In addition, analysis of whole blood for heavy metal contamination will be tested using AA or a similar technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIÑA‚ C.I.; SIROSKI‚ P.; LARRIERA‚ A.; LANCE‚ V.; VERDADE‚ L.M.. The temperature-sensitive period (TSP) during incubation of broad-snouted caiman (Caiman latirostris) eggs. Amphibia-Reptilia, v. 28, n. 1, p. 123-128, 2007. (05/01024-4)