Fourier transform spectroscopy applied for sepses microorganisms identification

• Grant number: 09/12230-5
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: March 05, 2010
• End date: March 19, 2010
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Airton Abrahao Martin
• Grantee: Airton Abrahao Martin
• Visiting researcher: Dieter Naumann
• Visiting researcher institution: Robert Koch Institute (RKI), Germany
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

Professor Dr. Dieter Naumann is the head of the Department of Biomedical Spectroscopy at the Robert Koch Institute. Prof. Naumann was the pioneered for the use of FT-IR spectroscopy in the identification of microorganism. Currently he has more than 180 indexed articles in the filed and its h-factor is extremely high. His visit in our laboratory is linked to the master's thesis of two students, as well as to developing a more close collaboration between our research groups with the exchange of students. On this trip, two projects should be addressed: identification of microorganisms that cause sepsis and infrared spectroscopy characterization and identification of pathogens of periodontal disease by optical spectroscopy. The goals for the first project is (a) Characterize the main organisms isolated from septic patients and (b) identify and distinguish microorganisms from patients with signs of sepsis by infrared spectroscopy. In the second project, the goal is the identification and characterization by optical spectroscopy, from standard cell American Type Culture Collection (ATCC) and clinical cells of microorganisms involved in bacterial etiology of periodontal disease. Assess and quantify the spectral similarities between biochemical components of clinical cell and cells ATCC. We will work together on the following work packages: We will construct and test a device for the accurate transfer of microbial micro colonies from solid nutrient agar plates to infrared substrates and we will measure these micro colony imprints with your FT-IR microscope and will define spectral quality criteria and criteria for reproducibility of measurements (AU)