Study of bacterial action and their toxins in inflammatory cytokine production secreted by fibroblasts and odontoblasts from endodontic infection: study in vitro

• Grant number: 12/09961-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Alexandre Augusto Zaia
• Grantee: Alexandre Augusto Zaia
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The dental pulp contains a heterogeneity of cells in its composition, among them stand out from the fibroblasts and odontoblasts. When this tissue is attacked by bacteria and / or their byproducts, odontoblasts and fibroblasts can secrete several inflammatory cytokines in order to attract and activate immune cells to contain infectious agents. IL-1 beta, TNF-alpha, IL-6 and IL-8 proteins are secreted by fibroblasts and odontoblasts mainly involved in the origin and activation of the inflammatory process. This study aims to analyze whether the contact of S. mutans , P. gingivalis, Neisseria spp. and / or its byproducts in vitro odontoblasts and fibroblasts stimulated with an increased production and secretion of inflammatory cytokines by these cells. The proteins are quantified by flow cytometry type CBA, and gene expression by Real Time - PCR. Subsequently, the findings will be subjected to specific statistical tests. (AU)