Fluorescence spectroscopy for biological systems studies and their applications at biomaterials development.

• Grant number: 12/13596-6
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Patricia Targon Campana
• Grantee: Patricia Targon Campana
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The understanding of molecular structure and protein function mechanisms is one of the most interesting challenges in Science due to the crucial role that these molecules play in all biological processes for life appearing and maintenance. In this way, elucidating the molecular mechanisms that can interfere on protein and peptide activities as a function as the changes promoted by external agents and specific bindings is essential to natural processes comprehension and for biomaterials development as biofunctionalized scaffolds, biosensors and biofuel cells. From these studies there are several spectroscopic methods that allowed not only the characterization of structural changes, but also the thermodynamic parameters determination for the biomolecules that are being studied. From the several physic-chemical known methods, steady-state fluorescence spectroscopy has been widely used at most biophysics and biomaterials studies by reason of the high sensitivity for studying changes at fluorophors vicinities in both intrinsic (aromatic amino acids) e and extrinsic. In some cases, is even possible monitor tertiary conformational changes of proteins and peptides. Hence, it is being proposed in this project the utilization of the steady-state fluorescence spectroscopy technique to enlighten the conformational changes in different biomolecules with application to biomaterials development. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPANA, PATRICIA T.; MARLETTA, ALEXANDRE; PIOVESAN, ERICK; FRANCISCO, KELLITON J. M.; NETO, FRANCISCO V. R.; PETRINI JR, LEANDRO; SILVA, THIAGO R.; MACHADO, DANILO; BASOLI, FRANCESCO; OLIVEIRA JR, OSVALDO N.; et al. Pulsatile Discharge from Polymeric Scaffolds: A Novel Method for Modulated Drug Release. BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, v. 92, n. 7, p. 1237-1244, JUL 2019. (10/06728-8, 12/13596-6, 13/14262-7)

MECHERI, BARBARA; DE PORCELLINIS, DIANA; CAMPANA, PATRICIA T.; RAINER, ALBERTO; TROMBETTA, MARCELLA; MARLETTA, ALEXANDRE; OLIVEIRA, JR., OSVALDO N.; LICOCCIA, SILVIA. Tuning Structural Changes in Glucose Oxidase for Enzyme Fuel Cell Applications. ACS APPLIED MATERIALS & INTERFACES, v. 7, n. 51, p. 28311-28318, DEC 30 2015. (12/13596-6, 13/14262-7)