Mechanisms of toxicity of fipronil in mitochondria and rat isolated hepatocytes

• Grant number: 12/15135-6
• Support Opportunities: Regular Research Grants
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Fábio Erminio Mingatto
• Grantee: Fábio Erminio Mingatto
• Host Institution: Universidade Estadual Paulista (UNESP). Campus Experimental de Dracena. Dracena , SP, Brazil

Abstract

The liver plays central role in metabolism, because it receives nutrients and xenobiotics that are absorbed by it, processed, stored and released into the blood and produce and store bile. The activities of biotransformation of xenobiotics are performed mainly by the liver by cytochrome P450. Most of these are associated with detoxification reactions, but they may represent a major problem because the metabolites produced may be highly reactive and more toxic than the parent compound. Mitochondria are responsible for the synthesis of almost all the ATP required for maintenance of cell structure and function. The parenchymal cells (hepatocyte) is the main functional unit of the liver and has been used extensively to study the metabolism and toxicity of a great variety of chemicals, since it provides adequate characteristics to the study of damage to these substances and their metabolites can cause . Fipronil is an insecticide with broad-spectrum of action used extensively to control pests. There are reports in the literature poisoning caused by the compound, leading to death. Its toxic action is attributed to the ability of the compound to act on receptors of ³-aminobutyric acid as a noncompetitive blocker. However, its action is not restricted to the central nervous system, since there are reports of liver toxicity. Thus, it is very important to elucidate the mechanisms of hepatotoxicity of the compound to help in the treatment in cases of intoxication by mammals. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUELFI, MARIELI; MAIOLI, MARCOS ANTONIO; TAVARES, MARCO AURELIO; MINGATTO, FABIO ERMINIO. Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation. Brazilian Archives of Biology and Technology, v. 58, n. 6, p. 843-853, NOV-DEC 2015. (12/24872-4, 12/15135-6)

TAVARES, MARCO A.; PALMA, IVO D. F.; MEDEIROS, HYLLANA C. D.; GUELFI, MARIELI; SANTANA, ANDREIA T.; MINGATTO, FABIO E.. Comparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondria. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v. 40, n. 1, p. 206-214, JUL 2015. (12/15135-6)

DIAS DE MEDEIROS, HYLLANA CATARINE; CONSTANTIN, JORGETE; ISHII-IWAMOTO, EMY LUIZA; MINGATTO, FABIO ERMINIO. Effect of fipronil on energy metabolism in the perfused rat liver. Toxicology Letters, v. 236, n. 1, p. 34-42, JUL 2 2015. (12/21869-2, 12/15135-6)