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Poliphasic study for the identification of zearalenone and type B trichothecenes producing Fusarium spp. strains

Grant number: 12/14157-6
Support Opportunities:Regular Research Grants
Start date: October 01, 2012
End date: December 31, 2014
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Benedito Corrêa
Grantee:Benedito Corrêa
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

F. graminearum, along with F. Culmorum and F. avenanceum is one of the main fungi species responsible for wheat infection. These fungi cause great yield lost due to plant and grain rot and the production of toxic secondary metabolites called mycotoxins. To create new efficient ways of combat, is essential profound knowledge of the epidemiological agent and its interaction host-plant. The Fusarium genus has high diversity and controversial taxonomy, which may lead to superficial or inconclusive identifications. Thus, the aim of this project is employing a polyphasic approach, including phenotypic (morphological and VCG- vegetative compatibility group) and genotypic characteristics (partial sequence of gene ef-1± and F.graminearum chemotypes) in the identification of Fusarium included in the teleomorf genus Gibberella, isolated from two wheat producers regions (RS and SP). F. Graminearum isolates will have their trichothecene production compared to TRI gene expression, involved in the trichothecenes production biosynthetic pathway. Also the wheat grains will be analized for type B trichothecene and zearalenone toxins. (AU)

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