Evaluation of human kallikrein 7 and superoxide dismutase-2 expression as biomarkers for cervical neoplasia

• Grant number: 12/16059-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Luiz Carlos Zeferino
• Grantee: Luiz Carlos Zeferino
• Host Institution: Centro de Atenção Integral à Saúde da Mulher (CAISM). Hospital da Mulher Professor Doutor José Aristodemo Pinotti. Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Silvia Helena Rabelo dos Santos

Abstract

The expression of kallikrein (hK7) and superoxide dismutase (SOD2) is present in carcinoma of the cervix, especially in more advanced cases. Levels and hK7 SOD2 increase with the severity of the lesions, which suggests that these proteins may be useful biomarkers for the severity of the cervical neoplastic lesions. Objective: To evaluate the variability of protein expression and SOD2 hK7 according to the severity of epithelial lesions of the cervix in cervical smears and histological sections in women. Methodology: The study design will be transversal and diagnostic test, and it will include 225 women who underwent conization or excision of the transformation zone by loop electrical excision procedure (LEEP). Two smears will be prepared immediately before the surgical procedure, one for cytological examination by conventional method and the other for the immunocytochemistry assay. The paraffin embedded tissue from LEEP specimens will be used in immunohistochemical assay. The protein expression of SOD2 hk7 and will be assessed through immunocytochemical and immunohistochemical assays. The immunochemical analysis will be categorized as positive or negative based on the appearance of cell clusters showing brown color. Expression present in more than 50% of the cells will be considered positive, for both proteins, hK7 and SOD2. The magnitude of association between two categorical variables will be estimated by odds ratio (OR) with confidence interval of 95% (95%). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TERMINI, LARA; BOCCARDO, ENRIQUE. Epithelial Organotypic Cultures: A Viable Model to Address Mechanisms of Carcinogenesis by Epitheliotropic Viruses. CURRENT TOPICS IN MEDICINAL CHEMISTRY, v. 18, n. 4, p. 246-255, 2018. (12/16059-1, 10/20002-0, 08/57889-1)

TALARICO, MARIA CECILIA RAMIRO; NUNES, RAFAELLA ALMEIDA LIMA; SILVA, GABRIELA AVILA FERNANDES; COSTA, LARISSA BASTOS ELOY DA; CARDOSO, MARCELLA REGINA; ESTEVES, SERGIO CARLOS BARROS; ZANATTA SARIAN, LUIS OTAVIO; ZEFERINO, LUIZ CARLOS; TERMINI, LARA. High Expression of SOD2 Protein Is a Strong Prognostic Factor for Stage IIIB Squamous Cell Cervical Carcinoma. ANTIOXIDANTS, v. 10, n. 5, MAY 2021. (12/16059-1, 10/07739-3, 15/26573-2, 08/57889-1)