Investigation of potential biomarkers for ventilator-associated pneumina in intensive care unit patients under invasive mechanical ventilation

• Grant number: 12/19536-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: November 30, 2014
• Field of knowledge: Health Sciences - Nursing
• Principal Investigator: Frederico Canato Martinho
• Grantee: Frederico Canato Martinho
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The search for inflammatory markers in the diagnosis of Ventilator-Associated Pneumonia (VAP) have increased over the last years. The objectives of this clinical study are: 1) To investigate and monitor the inflammatory profile in the lungs, blood circulation and oral cavity in patients from Intensive care unit patients (ICUP) requiring mechanical respiratory ventilation, by the levels of - IL-1±, IL-1², IL-6, IL-8, IL-10, TNF-± - and their antagonists - IL1-RA, IL-6 RA, sTNF- RI, sTNF-RII; 2) To investigate and monitor the inflammatory profile in the lungs, blood circulation and oral cavity in patients from ICUP requiring mechanical respiratory ventilation, by the levels of - MMP-1, MMP-2, MMP-8, MMP-9 - and their antagonists - TIMP-1, TIMP-2; 3) To investigate and monitor the levels of endotoxins profile in the lungs, blood circulation and oral cavity in patients from ICUP requiring mechanical respiratory ventilation by the LAL-assay (Limulus Amebocyte Lysate); 4) To investigate the biomarkers sTREM-1, prolactin, C-reactive protein in plasma samples in patients from ICUP requiring mechanical respiratory ventilation. Samples will be collected from the bronchoal-alveolar lavage fluid by an oral tracheal suction catheter, the blood circulation by using sterile "vacutainer" tubes containing heparin and the oral cavity (Crevicular fluid) using 3 sterile/ apyrogenic paper points, of 15 patients ICUP requiring mechanical respiratory ventilation. The levels of selected biomarkers and their antagonists will be determined by ELISA assay (Enzyme-Linked Immunosorbent Assay). The levels of endotoxins will be determined by the KQCL-test (LAL-assay). Data will be typed on a spreadsheet and statistical analyzed by SPSS for Windows and package Statistica 9.0. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINHO, FREDERICO C.; TALGE CARVATHO, CLAUDIO ANTONIO; OLIVEIRA, LUCIANE D.; FARIAS DE LACERDA, ANA JULIA; CARVALHO XAVIER, ANA CLAUDIA; AUGUSTO, MARINA GULLO; ZANATTA, RAYSSA FERREIRA; PUCCI, CESAR ROGERIO. Comparison of Different Dentin Pretreatment Protocols on the Bond Strength of Glass Fiber Post Using Self-etching Adhesive. JOURNAL OF ENDODONTICS, v. 41, n. 1, p. 83-87, JAN 2015. (12/19536-5)

MARTINHO, FREDERICO C.; GOMES, ANA P. M.; FERNANDES, ALETEIA M. M.; FERREIRA, NADIA S.; ENDO, MARCOS S.; FREITAS, LILIAN E.; CAMOES, IZABEL C. G.. Clinical Comparison of the Effectiveness of Single-file Reciprocating Systems and Rotary Systems for Removal of Endotoxins and Cultivable Bacteria from Primarily Infected Root Canals. JOURNAL OF ENDODONTICS, v. 40, n. 5, p. 625-629, MAY 2014. (12/19536-5)

XAVIER, ANA CLAUDIA C.; MARTINHO, FREDERICO C.; CHUNG, ADRIANA; OLIVEIRA, LUCIANE D.; JORGE, ANTONIO O. C.; VALERA, MARCIA C.; CARVALHO, CLAUDIO A. T.. One-Visit Versus Two-Visit Root Canal Treatment: Effectiveness in the Removal of Endotoxins and Cultivable Bacteria. JOURNAL OF ENDODONTICS, v. 39, n. 8, p. 959-964, AUG 2013. (12/19536-5)

MARTINHO, FREDERICO C.; LEITE, FABIO R. M.; NASCIMENTO, GUSTAVO G.; CIRELLI, JONI A.; GOMES, BRENDA P. F. A.. Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production. CLINICAL ORAL INVESTIGATIONS, v. 18, n. 9, p. 2095-2102, DEC 2014. (12/19536-5)