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Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of c-myc, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs

Grant number: 12/16068-0
Support type:Regular Research Grants
Duration: January 01, 2013 - June 30, 2015
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Renee Laufer Amorim
Grantee:Renee Laufer Amorim
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Canine prostate can be used as a model for the comparative study in prostatic disease in men. The dog and human are the only species that naturally develop prostatic carcinoma (PCa). Most of the animals models are tumors induced in isogenic animals. In this context, a natural model should be study and characterized, as a prognostic biomarker, clinical trial potential and as a progression model from pre neoplastic lesion to PCa, in the same manner as in men. Although the dog is considered the best natural model for the study of prostatic disease, similar to men, there are few studies in the literature that sustain that. So, more comparative studies should be done, in order to better characterize the PCa in dogs. The role of C-MYC oncogene and NKX3.1 and CDH1 tumoral suppressor are well established in human literature, as well as the methylation process of these two suppressor genes in human prostatic carcinoma. The aim of this project is to identify the correlation among C-MYC, NKX3.1 e E-cadherin protein expression and NKX3.1 and CDH1 methylation in pre neoplastic and PCa in dogs. Preview results from our group showed changes in these protein expression without copy number variations (gain or losses) in CGHarray, indicating the role of epigenetic changes in decrease protein expressio (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIVERA-CALDERON, LUIS G.; FONSECA-ALVES, CARLOS E.; KOBAYASHI, PRISCILA E.; CARVALHO, MARCIO; VASCONCELOS, ROSEMERI O.; LAUFER-AMORIM, RENEE. p-mTOR, p-4EBP-1 and eIF4E expression in canine prostatic carcinoma. Research in Veterinary Science, v. 122, p. 86-92, FEB 2019. Web of Science Citations: 1.
KOBAYASHI, PRISCILA E.; RODRIGUES, MARCELA M. P.; GARTNER, FATIMA; REMA, ALEXANDRA; FONSECA-ALVES, CARLOS E.; LAUFER-AMORIM, RENEE. Association between decreased expression of estrogen receptor alpha, androgen receptor and phosphatase and tensin homolog immunoexpression in the canine prostate. Pesquisa Veterinária Brasileira, v. 39, n. 1, p. 40-46, JAN 2019. Web of Science Citations: 0.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; RIVERA CALDERON, LUIS GABRIEL; FELISBINO, SERGIO LUIS; RINALDI, JAQUELINE DE CARVALHO; DRIGO, SANDRA APARECIDA; ROGATTO, SILVIA REGINA; LAUFER-AMORIM, RENEE. Immunohistochemical panel to characterize canine prostate carcinomas according to aberrant p63 expression. PLoS One, v. 13, n. 6 JUN 12 2018. Web of Science Citations: 4.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; LAUFER-AMORIM, RENEE. Evaluation of NKX3.1 and C-MYC expression in canine prostatic cancer. Research in Veterinary Science, v. 118, p. 365-370, JUN 2018. Web of Science Citations: 6.
KOBAYASHI, PRISCILA E.; FONSECA-ALVES, CARLOS E.; RIVERA-CALDERON, LUIS G.; CARVALHO, MARCIO; KUASNE, HELLEN; ROGATTO, SILVIA R.; LAUFER-ARNORIM, RENEE. Deregulation of E-cadherin, beta-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes. Research in Veterinary Science, v. 118, p. 254-261, JUN 2018. Web of Science Citations: 6.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILLA EMIKO; PALMIERI, CHIARA; LAUFER-AMORIM, RENEE. Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate. BMC Veterinary Research, v. 13, DEC 6 2017. Web of Science Citations: 8.
RIVERA-CALDERON, LUIS GABRIEL; FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; CARVALHO, MARCIO; DRIGO, SANDRA APARECIDA; VASCONCELOS, ROSEMERI DE OLIVEIRA; LAUFER-AMORIM, RENEE. Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis. Research in Veterinary Science, v. 106, p. 56-61, JUN 2016. Web of Science Citations: 10.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; RIVERA-CALDERON, LUIS GABRIEL; LAUFER-AMORIM, RENEE. Evidence of epithelial-mesenchymal transition in canine prostate cancer metastasis. Research in Veterinary Science, v. 100, p. 176-181, JUN 2015. Web of Science Citations: 7.
FONSECA-ALVES, CARLOS E.; RODRIGUES, MARCELA M. P.; DE MOURA, VERIDIANA M. B. D.; ROGATTO, SILVIA R.; LAUFER-AMORIM, RENEE. Alterations of C-MYC, NKX3.1, and E-Cadherin Expression in Canine Prostate Carcinogenesis. MICROSCOPY RESEARCH AND TECHNIQUE, v. 76, n. 12, p. 1250-1256, DEC 2013. Web of Science Citations: 15.

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