Proteomic analysis of adhesion of Escherichia coli associated with human sepsis (SEPEC) in human endothelial cells (HUVEC)

• Grant number: 12/51099-4
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: November 30, 2014
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Tomomasa Yano
• Grantee: Tomomasa Yano
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Proteomic analysis combined with molecular studies about virulence properties of pathogenic bacteria has provided numerous resources to understand the mechanisms by which microorganisms develop pathological processes in humans. Preliminary studies showed that all (100%) samples of Escherichia coli associated with human sepsis (SEPEC) adhered and invaded Vero cells (African green monkey kidney cells) and human endothelial cells (HUVEC), but these phenotypic characteristics were unrelated to the genotypic characteristics observed for SEPEC. Our data on genotypic and phenotypic characteristics of adhesion of SEPEC showed that this mechanism was not dependent on type I fimbriae, because fimH+ SEPEC adhered in the presence and absence of D-mannose (100%). For this reason we intend to identify, through a proteomic approach, the outer membrane proteins involved in adhesion of SEPEC to human endothelial cells (HUVEC) in the presence of D-mannose. (AU)