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Evaluation of passive immunity against rotavirus disease in calves born from vaccinated cows with commercial vaccine


This study will evaluate the effects of immunization of pregnant cows with inactivated commercial vaccine for rotavirus genotypes G6 and G10 in the transfer of colostral antibodies specific to newborn calves, the technique of indirect immunosorbent assay. To do so, will be tested samples of blood serum and colostrum of 26 Holstein cows, evenly distributed into two groups (immunized and not immunized G1 G2 - Control). Will be analyzed blood serum samples of calves born from cows that will form the experimental groups in seven different times: 0 hours - before the ingestion of colostrum at 24 hours to 48 hours to 7 days, 14 days, 21 days and 28 days of age. There will also be clinical follow-up of calves to verify the occurrence of diarrhea, in addition to research of rotavirus in feces of these animals, by electrophoresis in polyacrylamide gel and rnsaio immunoassay. The antibody titers of IgG isotypes, IgG1 and IgG2, IgM and Ig A in colostrum and blood serum of calves born from cows immunized and not immunized will be determined by the technique of enzyme immunoassay (EIA) Indirect double sandwich type. (AU)