Effect of a futsal match on lymphocyte activation, function and death

• Grant number: 12/20596-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2013
• End date: January 31, 2015
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Elaine Hatanaka Dermargos
• Grantee: Elaine Hatanaka Dermargos
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated researchers: Maria Fernanda Cury Boaventura ; Renata Gorjao

Abstract

We recently demonstrated that in competitive futsal matches, the cellular component of the players' innate immune system is affected, as evidenced by neutrophil dysfunction, and in its humoral components, by changes in acute phase proteins, cytokines and complement system proteins. Although it is known that high intensity exercise causes immunosuppression, the signaling mechanisms and their implications for the athlete's health are still uncertain. Thus, the purpose of this project is to study the activation, function and death of lymphocytes in athletes before and after a competitive futsal match. The number of leukocytes will be determined from hemograms. The occurrence of lymphocyte death will be assessed based on the cell viability assay, DNA fragmentation assay and expression of CD95 and phosphatidylserine by flow cytometry. The proliferative capacity of lymphocytes, production of reactive oxygen species and activation of expression/differentiation receptor (CD4, CD8, CD28, CD25, CTLA-4 and CD4/CD8 ratio will also be evaluated based on flow cytometry. The concentration of immunoglobulins A, G and M and the oxidative profile (antioxidant capacity, TBARS and LPO) in serum will be quantified. With these data, we intend to make an initial assessment of the mechanisms involved in immunosuppression after exercise and thus provide important data for interventions aimed at preventing infections arising after competitive matches, thereby improving the athlete's health and performance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CURY-BOAVENTURA, MARIA F.; GORJAO, RENATA; DE MOURA, NIVALDO R.; SANTOS, VINICIUS C.; BORTOLON, JOSE R.; MURATA, GILSON M.; BORGES, LEANDRO DA SILVA; MOMESSO, CESAR M.; DERMARGOS, ALEXANDRE; PITHON-CURI, TANIA C.; et al. The Effect of a Competitive Futsal Matchon T Lymphocyte Surface Receptor Signaling and Functions. FRONTIERS IN PHYSIOLOGY, v. 9, MAR 15 2018. (09/06039-0, 12/20596-2)