Expression of steroidogenic enzymes: Cytocromo P40 Aromatases, NADPH reductase and P450c17 during phases of reproductive development and spermatic segments in male captive-bred Spix's yellow-toothed and agouti

• Grant number: 12/11217-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2013
• End date: August 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Antônio Chaves de Assis Neto
• Grantee: Antônio Chaves de Assis Neto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Non-domestic species with great livestock potential should be operated in a rational way, in order to avoid extinction thereof. Thus is the importance of on reproduction researches with wild specimens bred in captivity. However, the Brazil still falls short when it comes to the creation of such as remunerable activity, despite the rich fauna diversity. In this context, exalt studies about various spermatogenic stages of development and the spermatic pathway components, especially those associated with puberty and sexual maturation should be performed. Enzymes of the cytochrome complex: P450 aromatase and P450c17 are responsible for estrogen production regulation and reproductive functions, as well as the estrogens and androgens metabolism. For studies, will be used testes and spermatic pathway fragments from agouti and cavies at different ages. The materials will come from Multiplication Center from Universidade Federal Rural do Semiarido, Mossoró, RN. The cellular and sub-surface analysis and photomicrographs will be conducted by Laboratory of transmission and scanning electron microscopy from FMVZ/USP. The gene expression identification and/or related-enzymes in steroidogenic process from various development stages will be made by immunohistochemical and real-time PCR. Will be made detailing from spermatic pathway by ultrastructure components to verify spermatogenic process development and other testicular compartments, correlated with the enzymes expression: Cytochrome P450 Aromatase, NADPH-Cytochrome P450 reductase and Cytochrome P450c17 (17-±-Hydroxylase/17,20-lyase) in agouti and cavies bred in captivity. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, P. R. S.; OLIVEIRA, M. F.; ARROYO, M. A. M.; SILVA, A. R.; RICI, R. E. G.; MIGLINO, M. A.; ASSIS NETO, A. C.. Ultrastructure of spermatogenesis in Spix's yellow-toothed cavy (Galea spixii). Reproduction, v. 147, n. 1, p. 13-19, JAN 2014. (10/14516-0, 12/11217-8)

SANTOS, P. R. S.; OLIVEIRA, F. D.; ARROYO, M. A. M.; OLIVEIRA, M. F.; CASTELUCCI, P.; CONLEY, A. J.; ASSIS NETO, A. C.. Steroidogenesis during postnatal testicular development of Galea spixii. Reproduction, v. 154, n. 5, p. 645-652, NOV 2017. (12/11217-8)

FRANCELIUSA DELYS DE OLIVEIRA; PAULO RAMOS DA SILVA SANTOS; MOACIR FRANCO DE OLIVEIRA; ANTÔNIO CHAVES DE ASSIS NETO. Galea spixii embryos have potential to produce steroid hormones. Animal Reproduction, v. 19, n. 4, 2023-01-16. (13/02535-9, 18/05952-3, 12/11217-8)

ARROYO, M. A. M.; SILVA, F. F. S.; SANTOS, P. R. S.; SILVA, A. R.; OLIVEIRA, M. F.; ASSIS NETO, A. C.. Ultrastructure of spermatogenesis and spermatozoa in agoutis during sexual development. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 29, n. 2, p. 383-393, 2017. (12/11217-8)