Distribution of nucleolar protein fibrillarin in the seminiferous epithelium of mammals and its role in the chromatoid bodies physiology

• Grant number: 12/22009-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2013
• End date: August 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Rita Luiza Peruquetti
• Grantee: Rita Luiza Peruquetti
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil
• Associated researchers: Maria Tercília Vilela de Azeredo Oliveira

Abstract

Chromatoid body (CB) is a typical cytoplasmic organelle of male germ cells, and it seems to be involved in RNA/protein accumulationfor later germ-cell differentiation. Most recent studies focused on the molecular compositionand function of the CB suggest that this structure acts as a subcellular coordinator of different RNA-processing pathways and centralizes posttranscriptional mRNA control in the cytoplasm of haploid germ cells. It has been hypothesized that nucleolar proteins, such as fibrillarin (B36), which is found in the nucleolar CFD playing a role in the rRNA metabolism, may be found in the CBs molecular composition. The aim of the present study is to follow the distribution of the nucleolar protein fibrillarin in seminiferous tubules in different stages of the spermatogenic cycle. We are also going to try to elucite the role that fibrillarin might play for the maintenance of CB physiology. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PERUQUETTI, RITA LUIZA. Perspectives on mammalian chromatoid body research. Animal Reproduction Science, v. 159, p. 8-16, AUG 2015. (13/14102-0, 13/26797-2, 12/22009-7)

SANTOS, ELISA G.; SILVA, MARAISA A.; AMORIM, RENATA P.; GIORDANO, LETICIA DE SOUZA; SILVA, DAYANA DE SALES; RASMUSSEN, LUCAS T.; PERUQUETTI, RITA L.. Aging and chromatoid body assembly: Are these two physiological events linked?. Experimental Biology and Medicine, v. 243, n. 11, p. 917-925, JUL 2018. (16/04580-0, 18/01554-3, 12/22009-7)

GARCIA, M. S.; ORCINI, W. A.; PERUQUETTI, R. L.; PEROBELLI, J. E.. New approach for reproductive toxicity assessment: chromatoid bodies as a target for methylmercury and polychlorinated biphenyls in prepubertal male rats. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 32, n. 10, p. 914-922, 2020. (16/04580-0, 13/14477-3, 13/26797-2, 12/22009-7)

AMORIM, R. P.; DAHER, I. P.; ARCANGELO, F. P. D.; BELLINI, M. F.; ORCINI, W. A.; SARRIA, G. R.; PERUQUETTI, R. L.. Short-term effects of green tea chronotherapy on the metabolic homeostasis of mice on different diets. Genetics and Molecular Research, v. 16, n. 2, MAY 16 2017. (14/07243-9, 12/22009-7)

DE PAULI, LARISSA FIAMENGUI; SANTOS, ELISA GOMES; DAHER ARCANGELO, FERNANDA PAZOTTI; ORCINI, WILSON APARECIDO; PERUQUETTI, RITA LUIZA. Differential expression of the nucleolar protein fibrillarin during mammalian spermatogenesis and its probable association with chromatoid body components. Micron, v. 94, p. 37-45, MAR 2017. (13/14102-0, 12/22009-7)