|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||January 01, 2016|
|Effective date (End):||February 28, 2017|
|Field of knowledge:||Biological Sciences - Zoology - Morphology of Recent Groups|
|Principal Investigator:||Maria Tercília Vilela de Azeredo Oliveira|
|Grantee:||Fernanda Fernandez Madeira|
|Home Institution:||Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil|
Triatomines are hematophagous insects of great importance to public health, since they are considered the main means of transmission of the protozoan Trypanosoma cruzi, etiologic agent of Chagas disease. Besides the epidemiological importance, these insects are of great relevance in cellular studies, for they present some peculiarities, such as holocentric chromosomes, inverted meiosis to the sex chromosomes and nucleolar persistence during meiosis. Nucleolar persistence is defined by the presence of the nucleolus or nucleolar corpuscles during every phase of meiosis. This phenomenon has been observed in 21 species of insects of the genera Triatoma, Rhodnius and Panstrongylus. However, the presence of this nuclear structure during every phase of meiosis does not mean the nucleolus is active, because although the nucleolus is present during every phase of spermatogenesis, it has been observed that it has no transcriptional activity and it is inactivated by epigenetic factors. Therefore, this project aims to describe the nucleologenesis in new species of at least seven new genera (Cavernicola, Psammolestes, Dipetalogaster, Eratyrus, Meccus, Mepraia, Nesotriatoma), in order to analyze whether nucleolar persistence is a synapomorphy of the Triatominae subfamily; as well as to analyze the nucleolar activity during meiosis in the species Triatoma infestans and Rhodnius montenegrensis using various cytochemical techniques, analysis by light microscopy and fluorescence microscopy, and by immunocytochemical analysis with confocal microscopy analysis, in order to characterize, by presence of RNA and fibrillarin nucleolar protein, whether there is transcriptional activity during the nucleolar persistence phenomenon.