Research Grants 12/51832-3 - Microbiologia oral, Saliva - BV FAPESP
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Analysis of a new Two Component System possibly involved in the adaptation of Streptococcus mutans and Streptococcus sanguinis to human saliva.

Grant number: 12/51832-3
Support Opportunities:Regular Research Grants
Start date: April 01, 2013
End date: March 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Renata de Oliveira Mattos Graner
Grantee:Renata de Oliveira Mattos Graner
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Streptococcus mutans (SM) and Streptococcus sanguinis (SS) are common bacterial species of the oral micro-biota of humans involved in the pathogenesis of dental caries. The greatest ecological niche of SM and SS are the teeth surfaces. While SM are considered a major caries pathogen, SS are classically defined as a beneficial species because it can inhibit the development of MS in dental biofilms. Both species are, however, opportunistic pathogens of infectious endocarditic promoted by bacteremia of oral origin. To be transmitted and occupy their ecological niches, these microorganisms must persist in saliva and adapt physiologically to the biophysical conditions of their niches, which vary at each stage of colonization. This complex process involves several changes in bacterial transcriptomes in response to environmental stimuli, which include salivary factors. Adaptation of bacterial transcriptomes to environmental stimuli is controlled by two-component systems (TCS). A typical TCS is comprised of a membrane receptor, typically a histidine kinase (HK), and an intracellular regulatory protein called response regulator (RR). The outer portion of the membrane receptor is activated by specific environmental stimuli, triggering phosphorylation in its intracellular portion. The phosphate group is then transferred to the cognate RR, which changes its conformation to bind to regulatory sequences of target genes. In this project, we intend to characterize a TCS with yet unknown function, identified in in silico analyses of the genome of strain S. mutans UA159, as an ortholog of the SptSR TCS (Spt of Saliva PersisTence) characterized in Streptococcus pyogenes. The SptSR TCS of S. pyogenes regulates diverse functions involved in virulence, adaptation and persistence in human saliva. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PALMA, THAIS H.; HARTH-CHU, ERIKA N.; SCOTT, JODIE; STIPP, RAFAEL N.; BOISVERT, HEIKE; SALOMAO, MARIANA F.; THEOBALDO, JESSICA D.; POSSOBON, ROSANA F.; NASCIMENTO, LEANDRO C.; MCCAFFERTY, JONATHAN W.; et al. Oral cavities of healthy infants harbour high proportions of Streptococcus salivarius strains with phenotypic and genotypic resistance to multiple classes of antibiotics. Journal of Medical Microbiology, v. 65, p. 9-pg., . (13/06820-0, 10/06785-1, 09/50547-0, 10/06786-8, 15/12940-3, 12/51832-3, 11/09761-9)
MATTOS-GRANER, RENATA O.; KLEIN, MARLISE I.; ALVES, LIVIA ARAUJO. The complement system as a key modulator of the oral microbiome in health and disease. CRITICAL REVIEWS IN MICROBIOLOGY, v. N/A, p. 30-pg., . (12/04222-5, 21/13074-9, 15/12940-3, 16/17216-4, 12/50966-6, 12/51832-3, 17/19899-4, 21/06801-1, 18/02054-4, 15/07237-1)
PALMA, THAIS H.; HARTH-CHU, ERIKA N.; SCOTT, JODIE; STIPP, RAFAEL N.; BOISVERT, HEIKE; SALOMAO, MARIANA F.; THEOBALDO, JESSICA D.; POSSOBON, ROSANA F.; NASCIMENTO, LEANDRO C.; MCCAFFERTY, JONATHAN W.; et al. Oral cavities of healthy infants harbour high proportions of Streptococcus salivarius strains with phenotypic and genotypic resistance to multiple classes of antibiotics. Journal of Medical Microbiology, v. 65, n. 12, p. 1456-1464, . (13/06820-0, 15/12940-3, 12/51832-3, 09/50547-0, 10/06786-8, 11/09761-9, 10/06785-1)
CAMARGO, TARSILA M.; STIPP, RAFAEL N.; ALVES, LIVIA A.; HARTH-CHU, ERIKA N.; HOFLING, JOSE F.; MATTOS-GRANER, RENATA O.. Novel Two-Component System of Streptococcus sanguinis Affecting Functions Associated with Viability in Saliva and Biofilm Formation. Infection and Immunity, v. 86, n. 4, . (15/12940-3, 09/50547-0, 15/07237-1, 12/51832-3, 12/04222-5)