Nanoscale titanium surface functionalization with GDF-5: in vitro and in vivo studies

• Grant number: 12/21330-6
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Paulo Tambasco de Oliveira
• Grantee: Paulo Tambasco de Oliveira
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Adalberto Luiz Rosa ; Cassio Edvard Sverzut ; Felippe José Pavinatto ; Osvaldo Novais de Oliveira Junior

Abstract

Initial studies from our research group have shown that the simple adsorption of the growth and differentiation factor 5 (GDF-5) on a titanium (Ti) surface nanotopography obtained by etching with sulfuric acid and hydrogen peroxide alters key parameters of the progression of osteogenic cell cultures, resulting in enhanced alkaline phosphatase (ALP) activity and matrix mineralization. To extend these observations, the present study aims at: 1) evaluating the effects of post-adsorption of the main plasma proteins albumin, fibrinogen and fibronectin on GDF-5-functionalized Ti nanotopography on the mineralization of osteogenic cell cultures; 2) comparing thin layer-by-layer (LbL) films of GDF-5 with inhomogeneous films of GDF-5 obtained by simple adsorption in terms of the osteogenic cell response, including mRNA expression of key osteoblast markers, ALP activity and matrix mineralization; 3) evaluating new bone formation in direct contact with, or adjacent to, nanostructured Ti implants functionalized with GDF-5, based on the results obtained with the in vitro experiments. (AU)