Analysis of osteogenesis, cytotoxicity and genotoxicity of bone substitutes in osteoblasts derived from human alveolar bone

• Grant number: 12/15650-8
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Samira Esteves Afonso Camargo
• Grantee: Samira Esteves Afonso Camargo
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The integration of the bone substitute results from material-cell interaction. The objective of this study is to investigate the behavior of human osteoblastic cells and osteogenesis in vitro on two types of bone substitute: a xenogenic - Bio-Oss ® (Geistlich) and other alloplastic - resorbable tissue replacement (RTR, Septodont). Data will be obtained as to adhesion, cell viability, total protein content, alkaline phosphatase activity, amount of nodules and matrix analysis of genotoxicity. The data will be evaluated by ANOVA and Tukey's multiple comparison statistical tests, both with a level significance of 5%. (AU)