Role of physical training in fetal reprogramming: emphasis on obesity

• Grant number: 13/04757-9
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2013
• End date: May 31, 2015
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Ronaldo de Carvalho Araújo
• Grantee: Ronaldo de Carvalho Araújo
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The increased prevalence of childhood obesity is alarming and epidemiological studies have suggested that early nutritional experiences may affect susceptibility to chronic diseases in adulthood, such as obesity, hypertension, cardiovascular disease and Type 2 diabetes and it is named metabolic programming. Changes in the fetal period may cause changes in lean body mass, endocrine, and vascular blood flow. Several studies demonstrating that epigenetic and metabolic programming may have a persistent effect on health and these can be transmitted to the next generations. Little is known about the role of exercise in modulating gene and is important to emphasize that the lack of physical activity and low levels of VO2 max are risk factors for developing Type 2 diabetes. From the information above we aimed to evaluate whether the exercise of pregnant mice can modulate the metabolism of offspring. Therefore, we will submit a group of female mice to a swimming protocol. After three weeks of adaptation to physical training five female mice will be mated with males of the same strain, untrained other 5 will also will be mated and the mothers in the control group. After the birth of offspring, we will evaluate the body of the puppies until the third month of age when they will be challenged with high fat diet for 16 weeks. After this period of induction of obesity metabolic variables, such as test insulin resistance, glucose uptake, cholesterol, triglycerides will be measured. The gene expressions related to metabolism and epigenetic changes involved will also be assessed.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WASINSKI, FREDERICK; ESTRELA, GABRIEL R.; ARAKAKI, ALINE M.; BADER, MICHAEL; ALENINA, NATALIA; KLEMPIN, FRIEDERIKE; ARAUJO, RONALDO C.. Maternal Forced Swimming Reduces Cell Proliferation in the Postnatal Dentate Cyrus of Mouse Offspring. FRONTIERS IN NEUROSCIENCE, v. 10, AUG 29 2016. (13/04757-9)

REIS, FELIPE C. G.; HARO, ANDERSON S.; BACURAU, ALINE V. N.; HIRABARA, SANDRO M.; WASINSKI, FREDERICK; ORMANJI, MILENE S.; MOREIRA, JOSE B. N.; KIYOMOTO, BEATRIZ H.; BERTONCINI, CLELIA R. A.; BRUM, PATRICIA C.; et al. Deletion of Kinin B2 Receptor Alters Muscle Metabolism and Exercise Performance. PLoS One, v. 10, n. 8, AUG 24 2015. (13/04757-9, 08/06676-8)

WASINSKI, FREDERICK; PEREIRA BACURAU, REURY FRANK; ESTRELA, GABRIEL RUFINO; KLEMPIN, FRIEDERIKE; ARAKAKI, ALINE MIDORI; BATISTA, ROGERIO OLIVEIRA; PAZELLO MAFRA, FERNANDO FRANCISCO; RIBEIRO DO NASCIMENTO, LUCAS FRANCISCO; HIYANE, MEIRE IOSHIE; VELLOSO, LICIO AUGUSTO; et al. Exercise during pregnancy protects adult mouse offspring from diet-induced obesity. NUTRITION & METABOLISM, v. 12, DEC 18 2015. (13/04757-9)