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Effect of viral oncoproteins E6 and E7 of HPV-16 in T cell response in patients with oropharyngeal squamous cell carcinoma

Grant number: 12/16269-6
Support type:Regular Research Grants
Duration: June 01, 2013 - May 31, 2015
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Glauco Issamu Miyahara
Grantee:Glauco Issamu Miyahara
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Assoc. researchers:Daniel Galera Bernabé ; Eder Ricardo Biasoli ; Fabio Daumas Nunes ; Sandra Helena Penha de Oliveira

Abstract

The Human Papillomavirus (HPV) has been associated with oropharyngeal squamous cell carcinoma as a possible risk factor. The viral oncoproteins E6 and E7 are capable of inhibit the production of some cytokines and also begin the production of others. So, the cellular response becomes deficient to the infection. The aim of this study is to evaluate the influence of the viral oncoproteins E6 and E7 of HPV-16 in the CD4+ T helper and CD8+ cells response in patients with oropharyngeal squamous cell carcinoma. The 40 participants will be divided in 20 patients diagnosed with oropharyngeal squamous cell carcinoma and 20 volunteers without cancer that will be submitted to the collect of tissue and peripheral blood samples to map the HPV-16 using Real Time PCR. T cells are isolated from peripheral blood, cultured in culture medium and stimulated by peptides E6 (16-40; 61-85; 91-115) e E7 (31-55; 46-70; 76-98) with 95% purity in three different concentrations. The level of the cytokines TNF±, IFN³, IL-2, IL-4 and IL-10 will be measured by ELISA technique in plasma and the culture medium of T cells after 1, 6 and 24 hours of stimulus. It will also investigate by immunohistochemical the effect of viral oncoproteins on lymphocyte proliferation, expression of p16 and characterization of tumor-associated inflammatory infiltrate. Data from different experiments will be analyzed for normality and then the results will be classified into parametric or nonparametric. (AU)