Evaluation of the effects of oil extracted from Acrocomia aculeata on reproductive toxicity induced by cyclophosphamide in male rats

• Grant number: 13/01802-3
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2013
• End date: June 30, 2015
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Arielle Cristina Arena
• Grantee: Arielle Cristina Arena
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Rafael Henrique Nóbrega ; Rozanna Marques Muzzi ; Silvia Aparecida Oesterreich ; Wilma de Grava Kempinas

Abstract

Cyclophosphamide (CFA) is extensively used as chemotherapeutic agent in clinical practice for the treatment of cancers. However, this compound causes several adverse effects including reproductive toxicity. As alternative to minimize these adverse effects, the CFA is normally used in combination with antioxidants. Acrocomia aculeata is a common species of palm savanna, known traditionally as food and medicinally to treat cardiovascular and inflammatory diseases. The oil pulp of A. aculeata has similar chemical composition to olive oil, indicating possesses bioactive compounds that can act as antioxidants. This study aims to evaluate if the oil extracted from this species can avoid or minimize reproductive toxicity induced by CFA. Adult male rats will be divided into 7 groups (n = 10): 1. Negative Control (V) treated with vehicle; 2. Positive Control (CFA): CFA-treated (5 mg/kg/day); 3. CFA + Beta-carotene (CB): treated with CFA (5 mg/kg/day) and Beta-carotene (10 mg/kg/day); 3. A. aculeata (A3): treated with oil pulp of A. aculeata (3 mg/kg/day); 4. A. aculeata (A30): treated with oil pulp of A. aculeata (30 mg/kg/day); 5. A3 + CFA: treated with CFA (5 mg/kg/day) and the oil pulp of A. aculeata (3 mg/kg/day) and 6. A30 + CFA: treated with CFA (5 mg/kg/day) and the oil pulp of A. aculeata (30 mg/kg/day). The treatments will be administered by gavage for 28 days. After treatment will be assessed the following parameters: hormonal dosage, sperm count, motility and morphology, histology of testis and epididymis and analysis of oxidative stress in the testis and epididymis and quantitative gene expression in real time in testiculars genes and evaluation of antioxidant activity in vitro. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARENA, A. C.; JORGE, B. C.; SILVA, M. C.; DE BARROS, A. L.; FERNANDES, A. A. H.; NOBREGA, R. H.; MARTINEZ, E. R. M.; CARDOSO, C. A. L.; ANSELMO-FRANCI, J. A.; MUZZI, R. M.. Acrocomia aculeata oil: Beneficial effects on cyclophosphamide-induced reproductive toxicity in male rats. ANDROLOGIA, v. 50, n. 6, AUG 2018. (13/01802-3)

DA SILVA, PERLA V. B.; RAMIRO, MARIANNE M.; IRIGUCHI, EDNA K. K.; CORREA, WILLIAM A.; LOWE, JENNIFER; CARDOSO, CLAUDIA A. L.; ARENA, ARIELLE C.; KASSUYA, CANDIDA A. L.; MUZZI, ROZANNA M.. Antidiabetic, cytotoxic and antioxidant activities of oil extracted from Acrocomia aculeata pulp. NATURAL PRODUCT RESEARCH, v. 33, n. 16, p. 2413-2416, AUG 18 2019. (13/01802-3)

TRAESEL, GISELI KARENINA; DE SOUZA, JULIANE COELHO; DE BARROS, ALINE LIMA; SOUZA, MARCOS ALEXANDRE; SCHMITZ, WANDERLEY ONOFRE; MUZZI, ROZANNA MARQUES; OESTERREICH, SILVIA APARECIDA; ARENA, ARIELLE CRISTINA. Acute and subacute (28 days) oral toxicity assessment of the oil extracted from Acrocomia aculeata pulp in rats. Food and Chemical Toxicology, v. 74, p. 320-325, DEC 2014. (13/01802-3)