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Screening Er:YAG (SSP) laser parameters to increase longitudinal adhesion to caries affected dentine

Grant number: 12/15141-6
Support Opportunities:Regular Research Grants
Duration: October 01, 2013 - November 30, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Adriana Bona Matos
Grantee:Adriana Bona Matos
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Patricia Moreira de Freitas Costa e Silva


The aim of this study is to investigate the effect of different Er: YAG laser (Super Short Pulse-SSP) parameters in sound and artificial caries-affected dentine. The flattened occlusal dentin of 275 human unerupted molars will be protected with acid resistant varnish in half of the occlusal surface (sound dentin - control) and on the other half caries affected dentine will be produced by microbiological challenge for 7 days. Thereafter different Er:YAG laser (SSP) surface conditioning parameters (Lightwalker AT, Fotona, Slovenija) will be applied (G1 - no conditioning; G2 - 80mJ, 2Hz, 50µs; G3 - 70mJ, 2Hz, 50µs; G4 - 60mJ, 2Hz, 50µs; G5 - 50mJ, 2Hz, 50µs). Distinct analysis will be accomplished through: Surface Microscopy (SEM) (n = 5), morphological evaluation of the bonding interface by SEM (n = 5), transmission electron microscopy (n = 5), ultramicrohardness test (n = 10) and microshear test (n = 20). A longitudinal analysis will be performed for all assays after 6 months storage, and these data will be compared with those obtained within 24 hours. The specimens will be assembled using a self-etching system (Clearfil SE Bond, Kuraray, Japan) associated to a composite resin (Z350 XT, 3M ESPE, USA). We intend to determine a conditioning parameter with Er:YAG (SSP) laser in sound and caries affected dentine in order to improve adhesion and its durability. The quantitative data will be statistically analyzed (Q<0.05) and the qualitative comparison will be described in relation to control tissue. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TREVELIN, LIVIA TOSI; MARQUES, MARCIA MARTINS; CORREA ARANHA, ANA CECILIA; ARANA-CHAVEZ, VICTOR ELIAS; MATOS, ADRIANA BONA. Effect of super short pulse Er:YAG laser on human dentinScanning electron microscopy analysis. MICROSCOPY RESEARCH AND TECHNIQUE, v. 78, n. 6, p. 472-478, . (12/15141-6)
FERREIRA DA SILVA, BEATRIZ TOGORO; CESAR, PAULO FRANCISCO; DE FREITAS, PATRICIA MOREIRA; PALMA-DIBB, REGINA GUENKA; MATOS, ADRIANA BONA. Three-dimensional profilometric assessment of Er:YAG laser irradiated unsintered zirconia. Journal of Materials Science, v. 51, n. 15, p. 7266-7275, . (12/15141-6, 15/13571-1)

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