Photodynamic therapy associated with nanoprobes for treating cancer

• Grant number: 13/17404-7
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2013
• End date: May 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Leandro José Raniero
• Grantee: Leandro José Raniero
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Isabelle Ferreira ; Juliana Ferreira Strixino ; Maria Angelica Gargione Cardoso

Abstract

The project aims is to increase the efficiency of Breast Cancer treatment via Photodynamic Therapy. Photodynamic Therapy is an alternative form of cancer treatment that leads to less aggressive approach to the body. Unlike chemotherapy and radiotherapy treatments, photodynamic therapy offers good results without cumulative toxicity and it can be used in immunocompromised patients. This therapy is based on the interaction of a chemical photosensitizing (drug), activating light and molecular oxygen. In this context, the drug is inserted into the body and absorbed by all cells but it will be more concentrated in cancer cells as function of time. Then the altered region is illuminated with appropriated wavelength, leading to cascade reactions that results in cell death. Therefore, this drug is also accumulating in healthy cells at lower concentrations and there is a need to increase efficiency. Based on this need, this project aims to manufacture a drug carrier (a nanoparticle) functionalized with protein (epidermal growth factor) and a photosensitizer. The receiver of this protein is overexpressed in malignant breast tissue, and then the functionalized nanoparticles will accumulate in these cells. The tissue irradiation will activate the photosensitizer in the functionalized nanoparticles, leading to destruction of the tissue. The nanoprobes will be tested in cell culture. This proposal brings together the experiences of individual components in a multidisciplinary way, where knowledge of their areas will converge to the proposal success. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROST, NATHANNE C. V.; BROCA, FATIMA M.; GONCALVES, GIULIA C.; CANDIDO, MARCELA A.; CASTILHO, MAIARA L.; RANIERO, LEANDRO J.. Synthesis of Iron Oxide Nanoparticles Optimized by Design of Experiments. Brazilian Journal of Physics, v. 49, n. 1, p. 22-27, FEB 2019. (13/17404-7)

VIEIRA, L.; CASTILHO, M. L.; FERREIRA, I.; FERREIRA-STRIXINO, J.; HEWITT, K. C.; RANIERO, L.. Synthesis and characterization of gold nanostructured Chorin e6 for Photodynamic Therapy. Photodiagnosis and Photodynamic Therapy, v. 18, p. 6-11, JUN 2017. (13/17404-7)

JESUS, V. P. S.; RANIERO, L.; LEMES, G. M.; BHATTACHARJEE, T. T.; CAETANO JUNIOR, P. C.; CASTILHO, M. L.. Nanoparticles of methylene blue enhance photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 23, p. 212-217, SEP 2018. (13/17404-7)

CASTILHO, M. L.; HEWITT, K. C.; RANIERO, L.. FT-IR characterization of a theranostic nanoprobe for photodynamic therapy and epidermal growth factor receptor targets. SENSORS AND ACTUATORS B-CHEMICAL, v. 240, p. 903-908, MAR 2017. (13/17404-7)

BHATTACHARJEE, T. T.; CASTILHO, M. L.; DE OLIVEIRA, I. R.; JESUS, V. P. S.; HEWITT, K. C.; RANIERO, L.. FTIR study of secondary structure changes in Epidermal Growth Factor by gold nanoparticle conjugation. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1862, n. 3, p. 495-500, MAR 2018. (13/17404-7)

AGUIAR, J. C.; MITTMANN, J.; CAETANO JUNIOR, P. C.; RANIERO, L.. Using FT-IR spectroscopy for the identification of the T-cruzi, T-rangeli, and the L-chagasi species. Experimental Parasitology, v. 192, p. 46-51, SEP 2018. (09/09559-5, 13/17404-7)

COMPARATO FILHO, OLAVO O.; MORAIS, V, FLAVIA; BHATTACHARJEE, TANMOY; CASTILHO, MAIARA L.; RANIERO, LEANDRO. Rapid identification of Paracoccidioides lutzii and P-Brasiliensis using Fourier Transform Infrared spectroscopy. Journal of Molecular Structure, v. 1177, p. 152-159, FEB 5 2019. (13/17404-7, 09/09559-5)

CASTILHO, M. L.; VIEIRA, L. S.; CAMPOS, A. P. C.; ACHETE, C. A.; CARDOSO, M. A. G.; RANIERO, L.. The efficiency analysis of gold nanoprobes by FT-IR spectroscopy applied to the non-cross-linking colorimetric detection of Paracoccidioides brasiliensis. SENSORS AND ACTUATORS B-CHEMICAL, v. 215, p. 258-265, AUG 2015. (13/17404-7, 09/09559-5)