Exercise-induced modulation of angiotensina II responses in femoral veins of hypertensive rats 2K1C

• Grant number: 13/22655-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2014
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Cardiorenal Pharmacology
• Principal Investigator: Agnaldo Bruno Chies
• Grantee: Agnaldo Bruno Chies
• Host Institution: Faculdade de Medicina de Marília (FAMEMA). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). Marília , SP, Brazil
• Associated researchers: Raquel Fantin Domeniconi

Abstract

In a previous study, we observed that the Ang II-induced contractions are very discreet in femoral veins, probably due to an effective and continuous modulation exerted by the locally produced nitric oxide (NO). In preparations taken from animals subjected to acute exercise or physical training, vasodilator prostanoids and endothelin-1 (ET-1), acting through ET-1 receptors subtype B (ETB), cooperate with the NO in the modulation of these Ang II responses. Possibly, these Ang II responses in femoral vein should be modulated during exercise to prevent an excessive resitance to the centripetal flow. Because of this, it is pertinent to know how the Ang II responses are modulated in pathophysiological conditions that involve elevations in serum/tissue Ang II, as in the premature phase of renovascular hypertension 2 kidneys - 1 clip (2K1C). Thus, this project proposes to study the Ang II responses in rat femoral vein taken from 2K1C rats, as well as the effects of exercise on these responses. In this manner, the angiotensin II and ET-1 functional responses will be studied in preparations of femoral vein obtained from the femoral vein 2K1C (kept at rest or exposed to acute exercise or training). These experiments will be performed also in the presence of inhibitors of nitric oxide synthase (NOS) and cyclooxygenase (COX), as well as in the presence of antagonists of ETA or ETB receptors and tempol (antioxidant). Simultaneously, the local expression of NOS-3, COX1, COX2 and NADPHoxidase (by Western blotting) and the ET-1 mRNA (by real time PCR) will be measured in these preparations. Finally, the lipid peroxidation will also be quantified in these veins. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHIES, AGNALDO BRUNO; SPADELLA, MARIA ANGELICA; DE OLIVEIRA, PRISCILA RAMOS; DOMENICONI, RAQUEL FANTIN; DE MELLO SANTOS, TALITA; MOREIRA, ROSELI PERES; ROSALES, CARLA B.; CASARINI, DULCE ELENA; NAVAR, LUIS GABRIEL. xercise-Induced Modulation of Angiotensin II Responses in Femoral Veins From 2-Kidney-1-Clip Hypertensive Rat. FRONTIERS IN PHYSIOLOGY, v. 12, APR 7 2021. (13/22655-9)

MUNHOZ, GUILHERME COSTA; ARRUDA, GUSTAVO VIANI; MADEIRA LANA SOARES, MARCELA MARIS; BATISTA, DAYANNE GUTTMANN; CHIES, AGNALDO BRUNO. Plasma antioxidant substances apparently do not influence the radiodermatitis occurrence. SCIENTIA MEDICA, v. 30, n. 1, p. 14-pg., 2020-09-17. (13/22655-9)

CHIES, AGNALDO BRUNO; SPADELLA, MARIA ANGELICA; DE OLIVEIRA, PRISCILA RAMOS; DOMENICONI, RAQUEL FANTIN; DE MELLO SANTOS, TALITA; MOREIRA, ROSELI PERES; ROSALES, CARLA B.; CASARINI, DULCE ELENA; NAVAR, LUIS GABRIEL. Exercise-Induced Modulation of Angiotensin II Responses in Femoral Veins From 2-Kidney-1-Clip Hypertensive Rats. FRONTIERS IN PHYSIOLOGY, v. 12, p. 11-pg., 2021-04-07. (13/22655-9)

DE OLIVEIRA, PRISCILA RAMOS; DE OLIVEIRA, PRISCILLA BIANCA; ROSSIGNOLI, PATRICIA DE SOUZA; SPADELLA, MARIA ANGELICA; CHIES, AGNALDO BRUNO. Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats. Experimental Physiology, v. 105, n. 4, FEB 2020. (13/22655-9)