Does desflurane anesthesia associated or not with nitrous oxide induce genotoxicity and oxidative stress in patients?

• Grant number: 13/16842-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: October 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Jose Reinaldo Cerqueira Braz
• Grantee: Jose Reinaldo Cerqueira Braz
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Camila Renata Corrêa ; Chung-Yen Chen ; Leandro Gobbo Braz ; Mariana Gobbo Braz ; Norma Sueli Pinheiro Módolo

Abstract

Our research group has been evaluating the genotoxic effect of the anesthetics utilized in patients. Desflurane and sevoflurane are the newest halogenated anesthetics, and desflurane has recently been used in Brazil. Little is known about the possible effects of this agent in relation to DNA damage and oxidative stress in patients undergoing surgery. Moreover, it is not yet known whether the concomitant use of nitrous oxide (N2O) with desflurane can induce additional toxic effects in patients. Thus, the current study will be conducted in 40 patients of both sexes, ASA I physical status, aged 18-50 years. Patients undergoing minimally invasive surgery lasting at least 90 min will be randomly allocated into two groups of 20 under desflurane associated or not with N2O (60%). Blood samples will be collected before anesthesia induction (baseline-T0), 90 min after the beginning of anesthesia (T1) and one day after surgery (T2). The basal and oxidative DNA damage will be detected by the comet assay, and oxidative stress will be evaluated by lipoperoxidation markers and protein carbonyls (immunoassay), and by total antioxidant performance (TAP) by fluorometry. It is expected that the findings may contribute to the acknowledgement about the possible mechanisms involved in genotoxicity and oxidative stress induced by desflurane and better understand the effect of N2O, when associated with desflurane. Thus, our study may contribute to our daily practices and ways of thinking what is the best choice for our patients. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARRUDA, NAYARA M.; BRAZ, LEANDRO G.; NOGUEIRA, FLAVIA R.; SOUZA, KATINA M.; AUN, ALINE G.; DE CARVALHO, LIDIA R.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Gene and systemic inflammatory effects and neuroendocrine response in surgical patients anesthetized with desflurane-nitrous oxide or desflurane-nitrous oxide-free: A randomized trial. International Immunopharmacology, v. 90, JAN 2021. (13/16842-0)

ARRUDA, NAYARA M.; BRAZ, LEANDRO G.; NOGUEIRA, FLAVIA R.; SOUZA, KATINA M.; AUN, ALINE G.; FIGUEIREDO, DRIELLE B. S.; LARA, JULIANA R.; SILVA, MARIANE APARECIDA P.; GOLIM, MARJORIE A.; DE CARVALHO, LIDIA R.; et al. Inflammation and DNA damage induction in surgical patients maintained with desflurane anesthesia. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 846, OCT 2019. (13/16842-0)

NOGUEIRA, FLAVIA R.; BRAZ, LEANDRO G.; SOUZA, KATINA M.; AUN, ALINE G.; ARRUDA, NAYARA M.; CARVALHO, LIDIA R.; CHEN, CHUNG-YEN O.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Comparison of DNA Damage and Oxidative Stress in Patients Anesthetized With Desflurane Associated or Not With Nitrous Oxide: A Prospective Randomized Clinical Trial. ANESTHESIA AND ANALGESIA, v. 126, n. 4, p. 1198-1205, APR 2018. (13/16842-0)

NOGUEIRA, FLAVIA R.; BRAZ, LEANDRO G.; DE ANDRADE, LEONARDO R.; DE CARVALHO, ANA LYGIA R.; VANE, LUIZ A.; MODOLO, NORMA SUELI P.; AUN, ALINE G.; SOUZA, KATINA M.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Evaluation of genotoxicity of general anesthesia maintained with desflurane in patients under minor surgery. Environmental and Molecular Mutagenesis, v. 57, n. 4, p. 312-316, MAY 2016. (13/16842-0)