Palatal mucosa derived fibroblasts present an adaptative behavior regarding cytokine secretion when grafted on the gingival margin

• Grant number: 14/04091-3
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: April 01, 2014
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Carlos Ferreira dos Santos
• Grantee: Carlos Ferreira dos Santos
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Considering that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted on the gingival margin regarding cytokine secretion. Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were cultured and stimulated withPorphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for 24 or 48 hours for comparative evaluation of the secretion of cytokines and chemokines, such as IL-6, IL-8/CXCL8, MIP-1±/CCL3, TGF-², VEGF and CXCL16. Cells were tested for viability through MTT assay, and secretion of these mediators was evaluated in the cell supernatant by Enzyme-Linked Immunosorbent Assay (ELISA). Fibroblasts from the palatal mucosa maintained the same secretion patternof IL-6 when grafted on the gingival margin. On the contrary, fibroblasts from the marginal gingival graft showed increased secretion of IL-8/CXCL8 even nonstimulated by LPS. Interestingly, MIP-1±/CCL3 secretion by fibroblasts from the marginal gingival graft was significantly increased after 48 hours of stimulation with Pg LPS and after 24 hours with Ec LPS. Only fibroblasts from the marginal gingivalgraft showed secretion of TGF-². VEGF and CXCL16 secretion by fibroblasts derived from both origins were not detected. Fibroblasts from the palatal mucosa seem to be adapted to local conditions of the site microenvironment when grafted to the gingival marginal area. This evidence supports their effective participation in the homeostasis of the marginal periodontium through secretion modulation of important inflammatory mediators. (AU)