Bioaccumulation and detoxification study of cyanobacteria toxins (microcystins and cilindrospermopsin) in lettuce and arugula

• Grant number: 14/01934-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2014
• End date: April 30, 2016
• Field of knowledge: Biological Sciences - Ecology - Ecosystems Ecology
• Principal Investigator: Maria Do Carmo Bittencourt de Oliveira
• Grantee: Maria Do Carmo Bittencourt de Oliveira
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Cyanobacteria are photosynthesizers organisms naturally occurring in aquatic ecosystems. However, the escalating eutrophication of these environments has been causing ecological unbalance and excessive growth of such organisms. Cyanobacteria are producers of several toxins, microcystins and cilindrospermopsins among them, which can induce intoxication and death in mammals, humans inclusively, additionally to the promotion of tumors. The present study aim at the investigation of microcystin (MC) and cilindrospermopsin (CYN) incorporation in leaf tissues of lettuce (Lactuca sativa L.) and arugula (Eruca sativa Mill.), as well as the following up of the detoxification process of cyanotoxins in leaf tissues all along 7 days. It is also our goal to compare results obtained with the analytical (LC-MS/MS) and immune-enzymatic (ELISA) methods. In order to check bioaccumulation followed by a posteriori detoxification of lettuces, MC and CYN solutions with concentrations regularly found in the aquatic ecosystems (up to 10.0 µg.L-1) will be applied during the ending cycle of cultures and over the adult lettuce leaves, by means of manual irrigation simulating a spraying device. It will be performed, at the same time, a bioaccumulation assay in lettuce using MC-LR and MC-RR equivalent found in crude extract from the Microcystis aeruginosa BCCUSP232 toxic strain. The MCs and CYN concentrations in the leaf tissues will be analyzed through Liquid Chromatography Coupled with Tandem Mass Spectrometry (LC-MS/MS). All experiments will be performed in triplicate. Our hypothesis are: (a) MC and CYN are bioaccumulated in the leaf tissues of lettuce and arugula ; (b) ingestion of these greenery could exceed the maximum human daily tolerable limit, this way entering the food chain with serious health harms; (c) once bioaccumulation takes place, the detoxification process of leaf tissues is triggered out variable periods, depending upon the plant species. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Cylindrospermopsin induced changes in growth, toxin production and antioxidant response of Acutodesmus acuminatus and Microcystis aeruginosa under differing light and nitrogen conditions. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 142, p. 189-199, AUG 2017. (13/11306-3, 13/15296-2, 15/17397-6, 14/26898-6, 14/01934-0)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; HEREMAN, TALITA CAROLINE; SASAKI, FABIANA FUMI; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Selective membrane permeability and peroxidase activity response of lettuce and arugula irrigated with cyanobacterial-contaminated water. ENVIRONMENTAL EARTH SCIENCES, v. 74, n. 2, p. 1547-1553, JUL 2015. (13/11306-3, 14/01934-0)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; LORENZI, ADRIANA STURION; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Does anatoxin-a influence the physiology of Microcystis aeruginosa and Acutodesmus acuminatus under different light and nitrogen conditions?. Environmental Science and Pollution Research, v. 23, n. 22, p. 23092-23102, NOV 2016. (13/11306-3, 14/26898-6, 14/01934-0, 13/15296-2)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Potential human health risk assessment of cylindrospermopsin accumulation and depuration in lettuce and arugula. HARMFUL ALGAE, v. 68, p. 217-223, SEP 2017. (13/11306-3, 14/01934-0, 15/17397-6)

BITTENCOURT-OLIVEIRA, MARIA DO CARMO; CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; DE TOLEDO ARRUDA-NETO, JOAO DIAS; DE OLIVEIRA, ENIO TIAGO; DOS SANTOS, FLAVIO. Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 128, p. 83-90, JUN 2016. (13/11306-3, 14/01934-0)

CORDEIRO-ARAUJO, MICHELINE KEZIA; CHIA, MATHIAS AHII; DE TOLEDO ARRUDA-NETO, JOAO DIAS; TORNISIELO, VALDEMAR LUIZ; VILCA, FRANZ ZIRENA; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Microcystin-LR bioaccumulation and depuration kinetics in lettuce and arugula: Human health risk assessment. Science of The Total Environment, v. 566, p. 1379-1386, OCT 1 2016. (13/11306-3, 14/01934-0, 15/17397-6)

CHIA, MATHIAS AHII; CORDEIRO-ARAUJO, MICHELINE KEZIA; BITTENCOURT-OLIVEIRA, MARIA DO CARMO. Growth and antioxidant response of Microcystis aeruginosa (Cyanobacteria) exposed to anatoxin-a. HARMFUL ALGAE, v. 49, p. 135-146, NOV 2015. (13/11306-3, 14/26898-6, 14/01934-0)