Effects of creatine supplementation in ischemia-reperfusion injury after unilateral lung transplantation in rats

• Grant number: 13/14848-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2014
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Rogerio Pazetti
• Grantee: Rogerio Pazetti
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Francine Maria de Almeida ; Rodolfo de Paula Vieira

Abstract

Ischemia-reperfusion (IR) injury is a complication that can raise the risk of death after lung transplantation, by activating the innate immune system to induce inflammation. This occurs when the supplementation of oxygen in the lungs is interrupted (ischemia) and is followed by reperfusion period. During ischemia, oxygen supply is below metabolic needs, resulting in depletion of cellular reserves of ATP and increased production of reactive oxygen species (ROS) and nitrogen (RNS). ROS and RNS are able to react and modify the molecular structures of lipids, carbohydrates, proteins and deoxyribonucleic acid, leading to structural and functional damage of the cells. Additionally, the IR also triggers an intense inflammatory process, characterized mainly by the presence of neutrophils and macrophages, which release numerous inflammatory mediators, perpetuating inflammation. The lock of each of these steps is an attempt to mitigate the injury, thereby preventing dysfunction of the organs involved in IR, especially the lungs.This knowledge has evolved along with the study of antioxidants capable of neutralizing the effects of ROS and RNS. Accordingly, supplementation with creatine (Cr) has been studied, and its benefits in cases of brain IR are assigned by favoring the increased levels of phosphocreatine (PCr), which facilitates the formation of adenosine triphosphate (ATP), promoting the maintenance of intracellular Ca2+ levels and discourage formation of ROS and therefore the inflammatory process.Therefore, in this study we aimed to verify whether creatine supplementation has beneficial effects against IR injury in a model of lung transplantation in rats. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, FRANCINE MARIA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; SOUZA, RENATO APARECIDO; PETRONI, RICARDO COSTA; SOTO, SONIA FATIMA; SORIANO, FRANCISCO GARCIA; CAMILLO DE CARVALHO, PAULO TARSO; ALBERTINI, REGIANE; DAMACENO-RODRIGUES, NILSA REGINA; QUIRINO SANTOS LOPES, FERNANDA DEGOBBI TENORIO; et al. Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury. JOURNAL OF HEART AND LUNG TRANSPLANTATION, v. 35, n. 2, p. 242-250, FEB 2016. (12/15165-2, 14/14604-8, 13/14848-1)

DE ALMEIDA, FRANCINE MARIA; BATTOCHIO, ANGELA SILVA; NAPOLI, JOAO PITHON; ALVES, KATIUSA ABREU; BALBIN, GRACE SUSANA; OLIVEIRA-JUNIOR, MANOEL; MORIYA, HENRIQUE TAKACHI; PEGO-FERNANDES, PAULO; VIEIRA, RODOLFO PAULA; PAZETTI, ROGERIO. Creatine supplementation attenuates inflammation in collateral lung after left lung transplantation. European Respiratory Journal, v. 54, p. 2-pg., 2019-09-28. (13/14848-1)

ALMEIDA, FRANCINE M.; BATTOCHIO, ANGELA S.; NAPOLI, JOAO P.; ALVES, KATIUSA A.; BALBIN, GRACE S.; OLIVEIRA-JUNIOR, MANOEL; MORIYA, HENRIQUE T.; PEGO-FERNANDES, PAULO M.; VIEIRA, RODOLFO P.; PAZETTI, ROGERIO. Creatine Supply Attenuates Ischemia-Reperfusion Injury in Lung Transplantation in Rats. NUTRIENTS, v. 12, n. 9, SEP 2020. (12/15165-2, 13/14848-1)