Metallothioneins in fish from Amazon Region: metallomic study associated to mercury

• Grant number: 13/21297-1
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2014
• End date: June 30, 2016
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Pedro de Magalhães Padilha
• Grantee: Pedro de Magalhães Padilha
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Luciana Francisco Fleuri ; Paulo dos Santos Roldan

Abstract

Fish is an important source of protein with high biological value, vitamins and minerals. However, this food is also a major route of human exposure to toxic contaminants such as methylmercury (MeHg). Thus, their accumulation in fish may predispose serious risks for human health. The high levels of mercury found in soil, sediments, fish and humans in the Brazilian Amazon have been studied extensively by the scientific community in recent decades, seeking to elucidate mechanisms of toxicity of mercury species. The mercury species bind preferentially to metallothioneins - proteins of molecular weight below 10 kDa, which are characterized by having sulfur-containing amino acid residues, such as the cysteine. This may mean that the bioavailable fraction of the mercury species in the different species of fish can be dependent on how they are related to proteins. View of the above, this proposal work seeks to optimize: procedures for 2D-PAGE electrophoresis for fractionation and identification of metallothionein responsible for the transport of mercury in fish samples collected at the reservoir from AHE JIRAU - Madeira River Basin; a method for determination of mercury by GFAAS or CVAFS in spots protein obtained; speciation methodologies of mercury in extracts of protein pellets obtained from samples of muscle and liver; characterization of metallothioneins mercury carriers by ESI-MS and finally the analysis of expression of these proteins to looking for a possible correlation with the incorporation of mercury in these fishes. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVECCI-MENDONCA, BRUNA; DE SOUZA VIEIRA, JOSE CAVALCANTE; DE LIMA, PAULA MONTEIRO; LEITE, ALINE LIMA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. Study of proteins with mercury in fish from the Amazon region. Food Chemistry, v. 309, MAR 20 2020. (14/02638-5, 16/19404-2, 13/21297-1)

BITTARELLO, ALIS CORREIA; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE PAULA ARAUJO, WELLINGTON LUIZ; BATAGLIOLI, IZABELA DA CUNHA; DA SILVA, JANAINA MACEDO; RABELO BUZALAF, MARILIA AFONSO; FLEURI, LUCIANA FRANCISCO; PADILHA, PEDRO DE MAGALHAES. Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region. Food Chemistry, v. 276, p. 247-254, MAR 15 2019. (16/19404-2, 14/02668-1, 13/21297-1)

DE QUEIROZ, JOAO VITOR; SOUZA VIEIRA, JOSE CAVALCANTE; BATAGLIOLI, IZABELA DA CUNHA; BITTARELLO, ALIS CORREIA; BRAGA, CAMILA PEREIRA; DE OLIVEIRA, GRASIELI; FEDERICI PADILHA, CILENE DO CARMO; PADILHA, PEDRO DE MAGALHES. Total Mercury Determination in Muscle and Liver Tissue Samples from Brazilian Amazon Fish Using Slurry Sampling. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 184, n. 2, p. 517-522, AUG 2018. (10/51332-5, 13/21297-1)

DE LIMA, PAULA MONTEIRO; VIEIRA, JOSE CAVALCANTE SOUZA; CAVECCI-MENDONCA, BRUNA; FLEURI, LUCIANA FRANCISCO; DE LIMA LEITE, ALINE; BUZALAF, MARILIA AFONSO RABELO; PEZZATO, LUIZ EDIVALDO; BRAGA, CAMILA PEREIRA; DE MAGALHAES PADILHA, PEDRO. Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 194, n. 1, p. 259-272, MAR 2020. (10/51332-5, 13/21297-1)

SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE OLIVEIRA, GRASIELI; LEITE, ALINE DE LIMA; DE QUEIROZ, JOAO VITOR; CAVECCI, BRUNA; BITTARELLO, ALIS CORREIA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. Identification of protein biomarkers of mercury toxicity in fish. ENVIRONMENTAL CHEMISTRY LETTERS, v. 15, n. 4, p. 717-724, DEC 2017. (13/21297-1, 14/02668-1)

SOUZA VIEIRA, JOSE CAVALCANTE; DE OLIVEIRA, GRASIELI; BRAGA, CAMILA PEREIRA; FERNANDES, MILENI DA SILVA; DE MORAES, PAULA MARTIN; RABELO BUZALAF, MARILIA AFONSO; SILVA DE OLIVEIRA, LINCOLN CARLOS; PADILHA, PEDRO DE MAGALHAES. Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 197, n. 2, p. 667-675, OCT 2020. (13/21297-1, 14/02668-1)

SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE OLIVEIRA, GRASIELI; FEDERICI PADILHA, CILENE DO CARMO; DE MORAES, PAULA MARTIN; ZARA, LUIZ FABRICIO; LEITE, ALINE DE LIMA; RABELO BUZALAF, MARILIA AFONSO; PADILHA, PEDRO DE MAGALHAES. Mercury Exposure: Protein Biomarkers of Mercury Exposure in Jaraqui Fish from the Amazon Region. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 183, n. 1, p. 164-171, MAY 2018. (12/24035-5, 14/02668-1, 13/21297-1)

DE QUEIROZ, JOAO VITOR; CAVECCI-MENDONCA, BRUNA; SOUZA VIEIRA, JOSE CAVALCANTE; MARTINS, RENATA APARECIDA; DE ALMEIDA ASSUNCAO, ANDREY SAVIO; CAVALLINI, NUBYA GONCALVES; DOS SANTOS, FELIPE ANDRE; PADILHA, PEDRO DE MAGALHAES. Metalloproteomic Strategies for Identifying Proteins as Biomarkers of Mercury Exposure in Serrasalmus rhombeus from the Amazon Region. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 199, n. 2, MAY 2020. (13/21297-1, 16/19404-2)

DE QUEIROZ, JOAO VITOR; SOUZA VIEIRA, JOSE CAVALCANTE; DE OLIVEIRA, GRASIELI; BRAGA, CAMILA PEREIRA; BATAGLIOLI, IZABELA DA CUNHA; DA SILVA, JANAINA MACEDO; DE PAULA ARAUJO, WELLINGTON LUIZ; PADILHA, PEDRO DE MAGALHAES. Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 187, n. 1, p. 291-300, JAN 2019. (13/21297-1, 16/19404-2)

DOS SANTOS, FELIPE ANDRE; CAVECCI, BRUNA; SOUZA VIEIRA, JOSE CAVALCANTE; FRANZINI, VANESSA PEZZA; SANTOS, ADEMIR; LEITE, ALINE DE LIMA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil. Archives of Environmental Contamination and Toxicology, v. 69, n. 2, p. 223-229, AUG 2015. (13/21297-1, 10/51332-5, 10/51506-3)

VIEIRA, JOSE C. S.; CAVECCI, BRUNA; QUEIROZ, JOAO V.; BRAGA, CAMILA P.; PADILHA, CILENE C. F.; LEITE, ALINE L.; FIGUEIREDO, WLLYANE S.; BUZALAF, MARILIA A. R.; ZARA, LUIZ F.; PADILHA, PEDRO M.. Determination of the Mercury Fraction Linked to Protein of Muscle and Liver Tissue of Tucunar, (Cichla spp.) from the Amazon Region of Brazil. Archives of Environmental Contamination and Toxicology, v. 69, n. 4, p. 422-430, NOV 2015. (12/24035-5, 13/21297-1, 10/51332-5)

CERBINO, M. R.; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, C. P.; OLIVEIRA, G.; PADILHA, I. F.; SILVA, T. M.; ZARA, L. F.; SILVA, JR., N. J.; PADILHA, P. M.. Metalloproteomics Approach to Analyze Mercury in Breast Milk and Hair Samples of Lactating Women in Communities of the Amazon Basin, Brazil. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 181, n. 2, p. 216-226, FEB 2018. (10/51332-5, 13/21297-1)