Abstract
The respiratory chain alternative enzymes constitute additional pathways to the mitochondrial oxidative phosphorylation (OXPHOS) system, in which oxygen consumption is uncoupled from ATP production. The expression of alternative oxidases and alternative NADH dehydrogenases from tunicates and fungi in the fruit fly Drosophila melanogaster, in the mouse and in cultured human cells has proven to be benign and to counteract deleterious effects of defective OXPHOS systems, such as the high production of reactive oxygen species. The observed benefits are in paradox with the fact that genes for alternative enzymes were lost independently, early in the evolution of Vertebrata and Arthropoda. We thus propose to investigate the functions of an alternative enzyme from Ciona intestinalis (Tunicata: Ascidiacea), the alternative oxidase (AOX), when expressed in transgenic fruitflies. These organisms will be challenged by thermal and dietetic stress conditions to study mitochondrial metabolism and to evaluate aerobic performance. We will use a variety of molecular biology techniques (including RNA-Seq and high resolution mass spectrometry) to investigate the changes in cell transcriptomics and mitochondrial proteomics, providing data about the physiological alterations caused by AOX and insights into the evolution of the mitochondrial alternative pathways in animals. (AU)
Scientific publications
(11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SALMINEN, TIINA S.;
CANNINO, GIUSEPPE;
OLIVEIRA, MARCOS T.;
LILLSUNDE, PAIVI;
JACOBS, HOWARD T.;
KAGUNI, LAURIE S.
Lethal Interaction of Nuclear and Mitochondrial Genotypes in Drosophila melanogaster.
G3-GENES, GENOMES, GENETICS,
v. 9,
n. 7,
p. 2225-2234,
JUL 2019.
Web of Science Citations: 1.
SAARI, SINA;
GARCIA, GEOVANA S.;
BREMER, KATHARINA;
CHIODA, MARINA M.;
ANDJELKOVIC, ANA;
DEBES, PAUL V.;
NIKINMAA, MIKKO;
SZIBOR, MARTEN;
DUFOUR, ERIC;
RUSTIN, PIERRE;
OLIVEIRA, MARCOS T.;
JACOBS, HOWARD T.
Alternative respiratory chain enzymes: Therapeutic potential and possible pitfalls.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,
v. 1865,
n. 4, SI,
p. 854-866,
APR 1 2019.
Web of Science Citations: 6.
CAMARGO, ANDRE F.;
CHIODA, MARINA M.;
RODRIGUES, ANA P. C.;
GARCIA, GEOVANA S.;
MCKINNEY, EMILY A.;
JACOBS, HOWARD T.;
OLIVEIRA, MARCOS T.
Xenotopic expression of alternative electron transport enzymes in animal mitochondria and their impact in health and disease.
Cell Biology International,
v. 42,
n. 6, SI,
p. 664-669,
JUN 2018.
Web of Science Citations: 1.
URBINATI, ISMAEL;
STAFUZZA, NEDENIA BONVINO;
OLIVEIRA, MARCOS TULIO;
SELEGUIM CHUD, TATIANE CRISTINA;
HIGA, ROBERTO HIROSHI;
DE ALMEIDA REGITANO, LUCIANA CORREIA;
DE ALENCAR, MAURICIO MELLO;
BUZANSKAS, MARCOS ELI;
MUNARI, DANISIO PRADO.
Selection signatures in Canchim beef cattle.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY,
v. 7,
MAY 5 2016.
Web of Science Citations: 8.
ALVES BATISTA, DIEGO FELIPE;
FREITAS NETO, OLIVEIRO CAETANO;
BARROW, PAUL ANDREW;
DE OLIVEIRA, MARCOS TULIO;
ALMEIDA, ADRIANA MARIA;
FERRAUDO, ANTONIO SERGIO;
BERCHIERI, JR., ANGELO.
Identification and characterization of regions of difference between the Salmonella Gallinarum biovar Gallinarum and the Salmonella Gallinarum biovar Pullorum genomes.
INFECTION GENETICS AND EVOLUTION,
v. 30,
p. 74-81,
MAR 2015.
Web of Science Citations: 8.