Fluorescent incoherent digital holographic microscopy applied to study of polymeric nano sheets

• Grant number: 13/25703-4
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: October 17, 2014
• End date: January 14, 2015
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Mikiya Muramatsu
• Grantee: Mikiya Muramatsu
• Visiting researcher: Jorge Octavio Ricardo Perez
• Visiting researcher institution: Universidad de Oriente, Cuba
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In this project there are being study polymeric nano sheets using an holographic fluorescence spectro microinterferometer based in Fresnel Incoherent Digital Holography, there are investigated different properties of polymeric nano sheets as surface fluorosfores concentration, spatial distribution, etc. The fluorescence Digital holographic Microscopy until now has being little exploited because of instrumentation complexities and for that only was passively by mean of confocal scanning techniques, which is expensive and complicated for extract 3D information, also it will be perfectionated the experimental set up, the software for holographic processing and it will be tested with known samples. There were selected stables structures like polymeric nano sheets with dynamic properties as lipidics pseudo phases. The results can help in future to develop application for morphologic ultra sensivel diagnostic of different diseases by mean of the fluorescence study and birefringence of Hemozoin crystals. (AU)