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Characterization of cardiomyocytes dynamics by traction force microscopy (TFM) and Speckle

Grant number: 14/22102-2
Support type:Regular Research Grants
Duration: March 01, 2015 - August 31, 2017
Field of knowledge:Physical Sciences and Mathematics - Physics - General Physics
Principal researcher:Adriano Mesquita Alencar
Grantee:Adriano Mesquita Alencar
Home Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:José Eduardo Krieger ; Mikiya Muramatsu


This is a multidisciplinary project between physics and medicine whose main focus is to characterize and understand the dynamics of three types of cardiac cells, or cardiomyocytes: (1) the human iPS, (2) the neonate of rat WT (wild type), and (3) neonate rat KO-CPR3. These cardiomyocytes are currently used as experimental models for research aimed at understanding of heart disease and heart regeneration. Basal dynamics, as well as the response to drugs, of cardiomyocytes will be assessed in the first instance by two techniques: Traction Force Microscopy (TFM) and speckle interferometry. The first technique measures the dynamics of the cell stress on a flexible substrate, looking the motion of nanoparticles incorporated into the substrate. While the second technique will evaluate the fluctuations in the optical path due to the pulsation of cardiomyocytes. These two techniques are complementary and will provide valuable information with regard to the field of stress, frequency and homogeneity of contractions of these cells. During the project we will incorporate two other techniques, Digital Holographic Microscopy and visualization and image analysis of actin fibers via fluorescent markers. The first technique will complement the Speckle and the second complements the TFM in understanding the structural arrangement of these cells. Recently obtained preliminary results of TFM with WT cells, motivated this study. Understanding the differences and the behavior of these cells in the presence of drugs will have a positive impact on research aimed at the regeneration of the heart. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BLOISE, ANTONIO CARLOS; DOS SANTOS, JENNIFER ADRIANE; DE BRITO, ISIS VASCONCELOS; BASSANEZE, VINICIUS; GOMES, LIGIA FERREIRA; ALENCAR, ADRIANO MESQUITA. Discriminating aspects of global metabolism of neonatal cardiomyocytes from wild type and KO-CSRP3 rats using proton magnetic resonance spectroscopy of culture media samples. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, SEP 2020. Web of Science Citations: 0.
DO AMARAL, JONATAS BUSSADOR; BLOISE, ANTONIO CARLOS; FRANCA, CAROLINA NUNES; PEREZ-NOVO, CLAUDINA; MACHADO-SANTELLI, GLAUCIA MARIA; ALENCAR, ADRIANO MESQUITA; PEZATO, ROGERIO. Alterations in cellular force parameters and cell projections in Nasal polyps-derived fibroblasts. AURIS NASUS LARYNX, v. 47, n. 1, p. 98-104, FEB 2020. Web of Science Citations: 0.
DORTA, MARCEL P.; DE BRITO, ISIS V.; PEREIRA, ALEXANDRE C.; ALENCAR, ADRIANO M. Quantification of alignment of vascular smooth muscle cells. Cytometry Part A, v. 93A, n. 5, p. 533-539, MAY 2018. Web of Science Citations: 2.
ALMEIDA, ALEXANDRE B.; GIOVAMBATTISTA, NICOLAS; BULDYREV, SERGEY V.; ALENCAR, ADRIANO M. Validation of Capillarity Theory at the Nanometer Scale. II: Stability and Rupture of Water Capillary Bridges in Contact with Hydrophobic and Hydrophilic Surfaces. Journal of Physical Chemistry C, v. 122, n. 3, p. 1556-1569, JAN 25 2018. Web of Science Citations: 4.
PONTUSCHKA, W. M.; GIEHL, J. M.; MIRANDA, A. R.; DA COSTA, Z. M.; ALENCAR, A. M. Effect of the Al2O3 addition on the formation of silver nanoparticles in heat treated soda-lime silicate glasses. Journal of Non-Crystalline Solids, v. 453, p. 74-83, DEC 1 2016. Web of Science Citations: 3.
ALENCAR, ADRIANO MESQUITA; AYRES FERRAZ, MARIANA SACRINI; PARK, CHAN YOUNG; MILLET, EMIL; TREPAT, XAVIER; FREDBERG, JEFFREY J.; BUTLER, JAMES P. Non-equilibrium cytoquake dynamics in cytoskeletal remodeling and stabilization. SOFT MATTER, v. 12, n. 41, p. 8506-8511, 2016. Web of Science Citations: 3.

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