Study comparing treatments with visible light and associated phthalocyanine ultraviolet light in associated riboflavin inactivation of Borrelia anserina and Leishmania chagasi

• Grant number: 14/03353-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2014
• End date: August 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Raimundo Souza Lopes
• Grantee: Raimundo Souza Lopes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Adivaldo Henrique da Fonseca ; Antonio Claudio Tedesco ; Cristiane Beck ; Daniela Marques Maciel Dabus ; Leizinara Gonçalves Lopes ; Mary Marcondes

Abstract

Photodynamic therapy is presented as a promising clinical option for the treatment of tumors, viruses and bacteria through the use of photosensitizers nontoxic. Phthalocyanines and riboflavin, when associated with light, are capable of intercalation and oxidation of DNA bases, possess advantageous photophysical properties featuring high absorption in the range of red and ultraviolet light spectrum. The avian borreliosis is an acute, septicemic disease caused by the bacterium Borrelia anserina, transmitted by Argas miniatus. Because of the propensity of the new system of poultry, excluding the use of antibiotics, returns the concern with the appearance vector, which may lead to the emergence of disease. Canine visceral leishmaniasis is an emerging zoonosis caused by Leishmania infantum chagasi, transmitted by arthropods and gender Lutzomia phlebotomum, the dog being the main reservoir. The study aims to determine if the blood of Gallus gallus domesticus parasitized with Borrelia anserina, treated by two different protocols, using visible light associated with phthalocyanine and ultraviolet light associated with riboflavin , when inoculated in vitro, its ability to maintain growth amid Barbour-Stoenner-Kelly and when inoculated in vivo, is capable of causing disease. And check the visible light effect associated with inactivation of phthalocyanine in Leishmania infantum chagasi in the blood of dogs. (AU)