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Evaluation of riboflavin efficacy under the action of ultraviolet light for inactivation of the Leishmania (Leishmania) chagasi in canine blood for transfusion and study haematologic and biochemical changes during the period of storage

Abstract

Provides safety during the transfer procedure of blood products is of greatest importance to transfusion medicine; this requires addition of a proper blood to minimize the occurrence of pathogen transmission. The present study aims to characterize the possible hematological and biochemical changes occurring during storage of canine blood samples collected in plastic bags containing an anticoagulant solution and preserver of blood, consisting of citrate phosphate dextrose adenine, known as CPDA- 1 and to investigate the efficacy of treatment with riboflavin and ultraviolet radiation on the inactivation of Leishmania chagasi. For this, the project will be divided into two phases. The first will be checked possible blood disorders, which occur during a period of 21 days of storage, called "storage lesion", comparing a group of blood collected from dogs infected with Leishmania chagasi, with an exchange group blood collected from healthy dogs. In a second step, the parasitized canine blood is subjected to treatment with riboflavin and ultraviolet light to verify the inactivation of haemoparasites. The possibility of blood parasitized lose the ability to produce the Leishmaniasis, after inactivation, will be assessed by indirect Immunofluorescence Assay (IFA), Real-Time quantitative Polymerase Chain Reaction (qPCR) and inoculation of susceptible animal (Hamster), trying to identify if the association of riboflavin with UV light, inactivated, reduced or completely eliminated the parasites. (AU)