Contributions to understanding of structure and evolution of sex chromosome systems using as models grashopper and cricket species

• Grant number: 14/11763-8
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2014
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Diogo Cavalcanti Cabral de Mello
• Grantee: Diogo Cavalcanti Cabral de Mello
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The analysis of experimental models for knowledge of sex chromosomes evolution progressively advance and currently the molecular cytogenetic and genomic data has been explored for this proposes. In crickets and in some grasshopper species wide diversity of sex chromosomes systems have been observed, occurring X0/XX, neo-XY/XX, neo-X1X2Y/X1X1X2X2 and X1X20/X1X1X2X2, originated by distinct chromosomal rearrangements between autosomes and sex chromosomes. Although these systems have been studied only through classical cytogenetics and no information are available concerning molecular composition of these chromosomes. Due the occurrence of distinct mechanisms, in distinct stages of differentiation, the crickets and grasshoppers could be good experimental model to the understanding of origin/diversification patterns of sex chromosomes. Thus, the objective of this work is to analyze the structure and evolutionary patterns of distinct sex chromosome systems based in the analysis of distinct repetitive DNAs, such multigene families, satellite DNAs, microsatellites, transposable elements, microdissection and chromosome painting, associated to genomic data obtained from completely sequenced genomes and analysis by immunolocalization. The model species present distinct sex chromosome systems, including X0, X1X20, neo-XY and neo-X1X2Y, being Eneoptera surinamensis (neo-X1X2Y) and Ronderosia bergi (neo-XY) the main biological models for the work. The possibility of the integration of genomic (such as, for example, whole sequenced genomes and isolation of sequences specifically from sex chromosomes) and chromosomal data (obtained from classical cytogenetics and physical chromosome mapping) became real with the advent of new technologies. The association of these data in ortopterans will allow a deeper knowledge related to structure, molecular composition and evolution of sex chromosomes in the group. These data will useful to the proposition of hypotheses that raise the origin/evolution of derived sex chromosomes and the test of them. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALACIOS-GIMENEZ, OCTAVIO MANUEL; DIAS, GUILHERME BORGES; DE LIMA, LEONARDO GOMES; KUHN, GUSTAVO CAMPOS E SILVA; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO CAVALCANTI. High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis. SCIENTIFIC REPORTS, v. 7, JUL 25 2017. (14/02038-8, 14/11763-8)

PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Repetitive DNA chromosomal organization in the cricket Cycloptiloides americanus: a case of the unusual X(1)X(2)0 sex chromosome system in Orthoptera. Molecular Genetics and Genomics, v. 290, n. 2, p. 623-631, APR 2015. (14/02038-8, 14/11763-8)

PALACIOS-GIMENEZ, O. M.; MARTI, D. A.; CABRAL-DE-MELLO, D. C.. Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers. CHROMOSOMA, v. 124, n. 3, p. 353-365, SEP 2015. (12/01421-7, 14/11763-8)

PALACIOS-GIMENEZ, OCTAVIO MANUEL; BARDELLA, VANESSA BELLINI; LEMOS, BERNARDO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Satellite DNAs are conserved and differentially transcribed among Gryllus cricket species. DNA Research, v. 25, n. 2, p. 137-147, APR 2018. (15/16661-1, 16/01506-3, 14/11763-8)

ANJOS, ALLISON; PALADINI, ANDRESSA; MARIGUELA, TATIANE C.; CABRAL-DE-MELLO, DIOGO C.. U1 snDNA chromosomal mapping in ten spittlebug species (Cercopidade, Auchenorrhyncha, Hemiptera). GENOME, v. 61, n. 1, p. 59-62, JAN 2018. (14/06226-3, 14/11763-8)

BARDELLA, VANESSA BELLINI; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Uncovering the molecular organization of unusual highly scattered 5S rDNA: The case of Chariesterus armatus (Heteroptera). Gene, v. 646, p. 153-158, MAR 10 2018. (14/11763-8)

SANTANDER, MYLENA D.; CABRAL-DE-MELLO, DIOGO C.; TAFFAREL, ALBERTO; MARTI, EMILIANO; MARTI, DARDO A.; PALACIOS-GIMENEZ, OCTAVIO M.; CASTILLO, ELIO RODRIGO D.. ew insights into the six decades of Mesa's hypothesis of chromosomal evolution in Ommexechinae grasshoppers (Orthoptera: Acridoidea. ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 193, n. 4, p. 1141-1155, DEC 2021. (14/11763-8)

XAVIER, CRISLAINE; SANTOS SOARES, ROGEAN VINICIUS; AMORIM, IGOR COSTA; CABRAL-DE-MELLO, DIOGO CAVALCANTI; DE MOURA, RITA DE CASSIA. Insights into the karyotype evolution and speciation of the beetle Euchroma gigantea (Coleoptera: Buprestidae). Chromosome Research, v. 26, n. 3, p. 163-178, SEP 2018. (14/11763-8)

MILANI, DIOGO; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. The U2 snDNA Is a Useful Marker for B Chromosome Detection and Frequency Estimation in the Grasshopper Abracris flavolineata. Cytogenetic and Genome Research, v. 151, n. 1, p. 36-40, 2017. (11/19481-3, 14/11763-8)

FERRETTI, ANA B. S. M.; MILANI, DIOGO; PALACIOS-GIMENEZ, OCTAVIO M.; RUIZ-RUANO, FRANCISCO J.; CABRAL-DE-MELLO, DIOGO C.. High dynamism for neo-sex chromosomes: satellite DNAs reveal complex evolution in a grasshopper. HEREDITY, v. 125, n. 3, p. 124-137, SEP 2020. (15/16661-1, 14/11763-8)

MILANI, DIOGO; RUIZ-RUANO, FRANCISCO J.; CAMACHO, JUAN PEDRO M.; CABRAL-DE-MELLO, DIOGO C.. Out of patterns, the euchromatic B chromosome of the grasshopper Abracris flavolineata is not enriched in high-copy repeats. HEREDITY, v. 127, n. 5, SEP 2021. (14/11763-8, 15/16661-1)

PALACIOS-GIMENEZ, OCTAVIO M.; MILANI, DIOGO; SONG, HOJUN; MART, DARDO A.; LOPEZ-LEON, MARIA D.; RUIZ-RUANO, FRANCISCO J.; CAMACHO, JUAN PEDRO M.; CABRAL-DE-MELLO, DIOGO C.. Eight Million Years of Satellite DNA Evolution in Grasshoppers of the Genus Schistocerca Illuminate the Ins and Outs of the Library Hypothesis. GENOME BIOLOGY AND EVOLUTION, v. 12, n. 3, p. 88-102, MAR 2020. (14/11763-8)

MARTI, EMILIANO; MILANI, DIOGO; BARDELLA, VANESSA B.; ALBUQUERQUE, LUCAS; SONG, HOJUN; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Cytogenomic analysis unveils mixed molecular evolution and recurrent chromosomal rearrangements shaping the multigene families on Schistocerca grasshopper genomes. Evolution, JUL 2021. (14/11763-8, 19/19069-7)

MENEZES, RODOLPHO S. T.; CABRAL-DE-MELLO, DIOGO C.; MILANI, DIOGO; BARDELLA, VANESSA B.; ALMEIDA, EDUARDO A. B.. The relevance of chromosome fissions for major ribosomal DNA dispersion in hymenopteran insects. JOURNAL OF EVOLUTIONARY BIOLOGY, v. 34, n. 9, AUG 2021. (16/21098-7, 19/19069-7, 15/02432-0, 18/09666-5, 14/11763-8)

MARTI, EMILIANO; MILANI, DIOGO; BARDELLA, VANESSA B.; ALBUQUERQUE, LUCAS; SONG, HOJUN; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. Cytogenomic analysis unveils mixed molecular evolution and recurrent chromosomal rearrangements shaping the multigene families on Schistocerca grasshopper genomes. Evolution, v. 75, n. 8, p. 2027-2041, AUG 2021. (14/11763-8, 19/19069-7)

ANJOS, ALLISON; PALADINI, ANDRESSA; EVANGELISTA, OLIVIA; CABRAL-DE-MELLO, DIOGO C.. Insights into chromosomal evolution of Cicadomorpha using fluorochrome staining and mapping 18S rRNA and H3 histone genes. JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 57, n. 2, p. 314-322, MAY 2019. (11/19481-3, 14/11763-8)

FERRETTI, ANA B. S. M.; RUIZ-RUANO, FRANCISCO J.; MILANI, DIOGO; LORETO, VILMA; MARTI, DARDO A.; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO C.. How dynamic could be the 45S rDNA cistron? An intriguing variability in a grasshopper species revealed by integration of chromosomal and genomic data. CHROMOSOMA, v. 128, n. 2, p. 165-175, JUN 2019. (15/16661-1, 14/11763-8)

PALACIOS-GIMENEZ, OCTAVIO M.; MILANI, DIOGO; LEMOS, BERNARDO; CASTILLO, ELIO R.; MARTI, DARDO A.; RAMOS, ERICA; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO C.. Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis. BMC Evolutionary Biology, v. 18, JAN 8 2018. (14/02038-8, 14/11763-8, 15/05246-3)

ALVES PICCOLI, MARIANI CRISTINA; BARDELLA, VANESSA BELLINI; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Repetitive DNAs in Melipona scutellaris (Hymenoptera: Apidae: Meliponidae): chromosomal distribution and test of multiple heterochromatin amplification in the genus. Apidologie, v. 49, n. 4, p. 497-504, AUG 2018. (14/11763-8)

ANJOS, ALLISON; LORETO, VILMA; CABRAL-DE-MELLO, DIOGO C.. Organization of some repetitive DNAs and B chromosomes in the grasshopper Eumastusia koebelei koebelei (Rehn, 1909) (Orthoptera, Acrididae, Leptysminae). COMPARATIVE CYTOGENETICS, v. 10, n. 2, p. 219-228, 2016. (14/06226-3, 14/11763-8)

PALACIOS-GIMENEZ, OCTAVIO M.; ROBERTO CARVALHO, CARLOS; FERRARI SOARES, FERNANDA APARECIDA; CABRAL-DE-MELLO, DIOGO C.. Contrasting the Chromosomal Organization of Repetitive DNAs in Two Gryllidae Crickets with Highly Divergent Karyotypes. PLoS One, v. 10, n. 12, DEC 2 2015. (14/02038-8, 14/11763-8)

BUENO, DANILO; PALACIOS-GIMENEZ, OCTAVIO MANUEL; ANDREA MARTI, DARDO; MARIGUELA, TATIANE CASAGRANDE; CABRAL-DE-MELLO, DIOGO CAVALCANTI. The 5S rDNA in two Abracris grasshoppers (Ommatolampidinae: Acrididae): molecular and chromosomal organization. Molecular Genetics and Genomics, v. 291, n. 4, p. 1607-1613, AUG 2016. (14/11763-8, 14/02038-8, 11/19481-3, 11/18028-3)

BARDELLA, VANESSA BELLINI; MARIN FERNANDES, JOSE ANTONIO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Chromosomal evolutionary dynamics of four multigene families in Coreidae and Pentatomidae (Heteroptera) true bugs. Molecular Genetics and Genomics, v. 291, n. 5, p. 1919-1925, OCT 2016. (14/11763-8)

CASTILLO, ELIO R. D.; TAFFAREL, ALBERTO; MARONNA, MAXIMILIANO M.; MARTA CIGLIANO, MARIA; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.; MARTI, DARDO A.. Phylogeny and chromosomal diversification in the Dichroplus elongatus species group (Orthoptera, Melanoplinae). PLoS One, v. 12, n. 2, FEB 28 2017. (14/02038-8, 14/11763-8)