Influence of hydrogen sulfide (H2S) on adhesion molecule expression and/or function of human neutrophil and eosinophil

• Grant number: 14/12909-6
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2014
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Heloisa Helena de Araujo Ferreira
• Grantee: Heloisa Helena de Araujo Ferreira
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Our previous results showed that H2S could be beneficial to airways allergic disease, as asthma, by reducing in vivo eosinophil and neutrophil migration to the lungs of sensitized mice after allergic challenge. In this research, H2S also showed to be able to avoid the increase of lipid peroxidation that provokes cellular damage in asthma, by improving lung antioxidant enzyme activity (superoxide dismutase, glutathione peroxidase and glutathione reductase). These data showed for the first time in the scientific literature that the beneficial effect of H2S on lung allergic inflammation by inhibiting leucocyte recruitment involves increase in antioxidant defenses in lung tissues. On the other hand, little is known about the influence of H2S on the starting mechanisms of inflammatory response, as the expression and function of adhesion molecules involved in leukocyte migration. The objectives of this work are 1) verify the influence of slow-releasing -GYY4137- and fast releasing H2S compound - NaHS- on adhesion in vitro to human lung microvascular endothelial culture cells (HMVEC) of eosinophils and neutrophils obtained from human healthy volunteers; 2) attest the effect of these donors on adhesion molecules expressed on eosinophils, neutrophils and HMVEC; 3) investigate whether these H2S donors modulates functionality of adhesion molecules expressed on these cells, by chemotaxis in vitro studies and 4) verify the effect of H2S donors GYY4137 and NaHS on eosinophil and neutrophil apoptosis in vitro. (AU)