AtRALF1 perception mechanisms: its receptors and the dissociation between ion fluxes and the negative regulation of celular expansion

• Grant number: 14/14487-1
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2014
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Daniel Scherer de Moura
• Grantee: Daniel Scherer de Moura
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

RALF signaling peptides negatively regulate cell elongation and inhibit root growth. The membrane protein FERONIA has been recently identified as AtRALF1 receptor, however, this same protein has already been described as positive regulator of cell expansion, playing a role in a brassinosteroid parallel signaling pathway. Arabidopsis has nine RALF isoforms with high homology with the original RALF isolated from tobacco. With exception of AtRALF4, all of them are capable of inducing alkalinization of the extracellular media and also of inhibiting root growth. Mutations in AtRALF4 were able to restore the inhibiting capacity without any effect on the alkalinization activity. Based on our own data we propose the existence of two AtRALF1 perception systems: one via FERONIA and another one via a different membrane protein. Both would be responsible for AtRALF1 perception, but the first would mediate the ion fluxes and the second the root inhibition effect. The existence of two pathways would allow for the dissociation of both effects, the alkalinization and the root inhibition and would reconcile the data on FERONIA since the its insensitivity for RALF is only partial. The goal of this project is to gain information to prove the above stated hypothesis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BLACKBURN, MATTHEW R.; HARUTA, MIYOSHI; MOURA, DANIEL S.. Twenty Years of Progress in Physiological and Biochemical Investigation of RALF Peptides(1)([OPEN]). Plant Physiology, v. 182, n. 4, p. 1657-1666, APR 2020. (14/14487-1)

GE, ZENGXIANG; BERGONCI, TABATA; ZHAO, YULING; ZOU, YANJIAO; DU, SHUO; LIU, MING-CHE; LUO, XINGJU; RUAN, HAO; GARCIA-VALENCIA, LILIANA E.; ZHONG, SHENG; et al. Arabidopsis pollen tube integrity and sperm release are regulated by RALF-mediated signaling. Science, v. 358, n. 6370, p. 1596-1599, DEC 22 2017. (14/14487-1)

DRESSANO, KEINI; CECILIATO, PAULO H. O.; SILVA, APARECIDA L.; GUERRERO-ABAD, JUAN CARLOS; BERGONCI, TABATA; ORTIZ-MOREA, FAUSTO ANDRES; BUERGER, MARCO; SILVA-FILHO, MARCIO C.; MOURA, DANIEL S.. BAK1 is involved in AtRALF1-induced inhibition of root cell expansion. PLOS GENETICS, v. 13, n. 10, OCT 2017. (11/17264-5, 12/13658-1, 14/14487-1)