| Grant number: | 14/12757-1 |
| Support Opportunities: | Regular Research Grants |
| Start date: | November 01, 2014 |
| End date: | October 31, 2016 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal Investigator: | Eneiva Carla Carvalho Celeghini |
| Grantee: | Eneiva Carla Carvalho Celeghini |
| Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated researchers: | Fabio Celidonio Pogliani |
Abstract
After semen deposition in the reproductive tract occurs an inflammatory response that causes a systemic reaction called acute phase response characterized by the production of acute phase proteins. In addition, the metabolic and hormonal profile of the animal influences their reproductive functions so the study of them can assist in the evaluation of herds with different productive and reproductive rates. The semen quality used in TAI is a point that interferes with fertilization. The changes in the uterine hemodynamic according to the events that occur in the animal organism. Thus, for this study, three experiments were designed. Experiment 1 aims to study the blood components to compare blood profile among cows that were inseminated or not and verify the changes that occur in the uterine hemodynamics according to the concentration of blood components. Serum and plasma of cows that were inseminated (GIA, n = 10) and cows that have not gone through the process of insemination shall be used (CG, n - = 10). Samples were collected 30 hours before IA, 4, 24, 48 and 168 hours after AI. Experiment 2 was developed with the aim of studying the effects of different qualities of semen on blood components and on uterine hemodynamics, and yet the relationship between the concentration of blood components and uterine hemodynamics. Serum and blood plasma were collected from 362 cows calved divided into three groups according to semen quality for the realization of TAI will be used: Good (n=121), medium (n=121) and regular (n=120). Samples were collected 30 hours before IA, 4 and 24 hours after AI. Still a sample of 108 animals underwent blood sampling at the time of pregnancy diagnosis, which was performed 30 days after TAI by transrectal ultrasonography. The experiment 3 aims to compare the blood profiles between animals that became pregnant and remained empty and check the changes that occur in the uterine hemodynamics according to the result of the IATF. The same samples will be used in Experiment 2, however, a retrospective study will be conducted and the experimental groups will be divided according to the result of the IATF (X heifer empty). In all experiments, the same time of blood collection, sonographic evaluations were performed in uterus mode color Doppler and spectral. The serum and plasma experiments 1, 2 and 3 will be subject to quantification of acute phase proteins and metabolic blood components using automatic biochemical analyzer (RX Daytona) and hormone dosage by radioimmunoassay technique. The variables will be submitted to PROC GLM of SAS (SAS, version 9.2, 2010) and will be analyzed by PROC MIXED using linear mixed model for repeated measures. Statistics differences will be considered when p<0.05. (AU)
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