Blood profile evaluation of pregnant and empty cows submitted to FTAI protocol with semen assessed by fluorescent probes

• Grant number: 11/22833-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: November 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Eneiva Carla Carvalho Celeghini
• Grantee: Bruna Marcele Martins de Oliveira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Soon after the semen deposition the female reproductive tract it have an inflammatory reaction. The acute inflammatory reaction is of short duration and cause a systemic reaction called the acute phase response that is characterized by the production of acute phase proteins. In addition, the metabolic and energetic profiles of the animal influences their reproductive functions, so that the study of them can assist in the evaluation of herds with different productive and reproductive rates. In addition to the female physiological conditions, the quality of semen used in FTAI is one of the points that interfere with fertilization. Thus it is important to establish the changes that occur in the female blood from the period prior to artificial insemination to the time of pregnancy diagnosis and also check if the deposition of semen from different qualities in the female reproductive tract causes systemic changes. The objective of this study is to assess blood components by checking for differences in the acute phase proteins, metabolic and energetic profiles between pregnant and empty cows and between animals that were inseminated with semen from different qualities and also find the points that differ so as to suggest hypotheses that may explain the failure in fertilization. Will be used 362 Nelore calved cows with proven health of the reproductive tract. The animals were divided into three groups according to semen quality: Good (n = 121), medium (n = 121) and regular (n = 120) and had blood samples collected by puncturing the jugular vein in three times: 30 hours before insemination, 4 and 24 hours after insemination. A sample (n = 108) also had samples collected at diagnosis of pregnancy (30 days after insemination). The serum and plasma samples were stored in triplicate and frozen at -80C for further assessment.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Bruna Marcele Martins de. Blood profile evaluation of pregnant and non pregnant cows subjected to TAI protocol with semen evaluated by fluorescent probes and their relationship with uterine hemodynamics. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.