Studies on ovarian and uterine physiology and improvement of Assisted Reproduction Technologies to increase reproductive efficiency in cattle

• Grant number: 18/03798-7
• Support Opportunities: Research Projects - Thematic Grants
• Start date: December 01, 2018
• End date: November 30, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Roberto Sartori Filho
• Grantee: Roberto Sartori Filho
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• City of the host institution: Piracicaba
• Associated researchers: Alexandre Vaz Pires ; Danila Barreiro Campos ; Dante Pazzanese Duarte Lanna ; Eneiva Carla Carvalho Celeghini ; José Renato Silva Gonçalves ; Ky G. Pohler ; Leonardo de França e Melo ; Lucas Oliveira e Silva ; Luiz Lehmann Coutinho ; Manoel Francisco de Sá Filho ; Marcelo Marcondes Seneda ; Milo Wiltbank ; Natália Picoli Folchini ; Pedro Leopoldo Jerônimo Monteiro Junior ; Rodrigo Lemos Olivieri Rodrigues Alves ; Taynara Jaqueline Barreiro da Silva
• Associated scholarship(s): 23/02557-4 - Studies of ovarian and uterine physiology and improvement of assisted reproduction technologies to increase reproductive efficiency in cattle, BP.PD; 21/09904-6 - Physiological and reproductive parameters of 14- to 16-month old Bos indicus heifers subjected to different circulating concentrations of progesterone and different ovulation induction protocols, BP.DR; 22/00543-3 - Validation of a chemiluminescent immunoassay protocol for measuring circulating progesterone in cattle, BP.IC; + associated scholarships 21/09924-7 - Effect of steroid hormones on the modulation of pituitary responsiveness and gonadotropin release in cattle, BP.DR; 20/10659-3 - STUDIES OF OVARIAN AND UTERINE PHYSIOLOGY AND IMPROVEMENT OF ASSISTED REPRODUCTIVE TECHNOLOGIES TO ELEVATE REPRODUCTIVE EFFICIENCY IN CATTLE - HORMONE ASSAYS, BP.IC; 19/26773-2 - Fertility of Nelore heifers submitted to FTAI protocols of 7 or 9 days, with PGF administered at different moments, BP.TT - associated scholarships

Abstract

Experiments will be performed to better understand physiological aspects related to ovulation and maintenance of pregnancy in bovine females submitted to artificial insemination (AI) or embryo transfer (ET). In addition, studies will evaluate strategies to increase efficiency and/or simplify the use of fixed-time artificial insemination (FTAI) or embryo production protocols. Twenty-six experiments will be performed within six main themes, three of them about reproductive physiology (1 - Mechanisms involved in the maintenance of pregnancy; 2 - Ovarian, uterine and endocrine changes in response to different treatments or hormonal concentrations; 3 - Ovarian dynamics and embryo quality in FTAI protocols) and other three focused on reproductive biotechnologies (4 - Reproductive efficiency in Nelore cows and heifers submitted to FTAI protocols; 5 - Reproductive efficiency in dairy cows submitted to FTAI protocols; 6 - In vivo embryo production in superovulated cows inseminated with refrigerated sexed spermatozoa), in both beef cattle and dairy cattle. In these experiments, approximately 20,000 animals will be used. We will be performing sperm evaluations of the sires used, ovarian and transrectal uterine ultrasound examinations, blood sampling for hormone assays, and uterine flushings (when appropriate) to study ovarian and uterine dynamics and functions, embryo quality and fertility. (AU)

Scientific publications

(22)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, RODRIGO L. O. R.; SILVA, MATEUS A.; CONSENTINI, CARLOS E. C.; SILVA, LUCAS O. E.; FOLCHINI, NATALIA P.; OLIVA, ABRAHAM L.; PRATA, ALEXANDRE B.; GONCALVES, JOSE RENATO S.; WILTBANK, MILO C.; SARTORI, ROBERTO. Hormonal combinations aiming to improve reproductive outcomes of Bos indicus cows submitted to estradiol/progesterone-based timed AI protocols. Theriogenology, v. 169, p. 89-99, JUL 15 2021. (18/03798-7, 18/14723-8)

BASAVARAJA, RAGHAVENDRA; DRUM, JESSICA N.; SAPULENI, JACKSON; BIBI, LONICE; FRIEDLANDER, GILGI; KUMAR, SAI; SARTORI, ROBERTO; MEIDAN, RINA. Downregulated luteolytic pathways in the transcriptome of early pregnancy bovine corpus luteum are mimicked by interferon-tau in vitro. BMC Genomics, v. 22, n. 1, JUN 16 2021. (18/03798-7)

MONTEIRO, PEDRO L. J.; CONSENTINI, CARLOS EDUARDO C.; ANDRADE, JOAO PAULO N.; BEARD, ADAM D.; GARCIA-GUERRA, ALVARO; SARTORI, ROBERTO; WILTBANK, MILO C.. Research on timed AI in beef cattle: Past, present and future, a 27-year perspective. Theriogenology, v. 211, p. 11-pg., 2023-08-27. (18/03798-7)

SARTORI, R.; CONSENTINI, C. E. C.; ALVES, R. L. O. R.; SILVA, L. O.; WILTBANK, M. C.. Review: Manipulation of follicle development to improve fertility of cattle in timed-artificial insemination programs. ANIMAL, v. 17, p. 11-pg., 2023-05-01. (18/03798-7)

SARTORI, ROBERTO; BALISTRIERI, MIRELA; OLIVEIRA E SILVA, LUCAS; CONSENTINI, CARLOS EDUARDO CARDOSO; DE FRANCA E MELO, LEONARDO; PONTES, GUILHERME CORREA DE SOUSA; GAITKOSKI, DOUGLAS. Pregnancy loss in cattle with emphasis on embryo transfer programs. ANIMAL REPRODUCTION, v. 22, n. 3, p. 14-pg., 2025-01-01. (18/03798-7)

ALVES, RODRIGO L. O. R.; E SILVA, LUCAS O.; CONSENTINI, CARLOS E. C.; CAVALCANTI, PATRICIA R.; BALISTRIERI, MIRELA; V. BARROS, GUSTAVO; NAGLE, EDGAR; SARTORI, ROBERTO. Original Reproductive outcomes of prepubertal Bos indicus beef heifers raised in a pasture-based feeding system submitted to ovulation induction strategies prior to a timed-artificial insemination protocol. Theriogenology, v. 230, p. 9-pg., 2024-09-21. (21/09904-6, 18/03798-7)

LOPES, ANA LUIZA M.; KOLLING, GISELLY B.; SILVA, LUCAS O. E; ALVES, RODRIGO L. O. R.; DE ASSUMPCAO, FABIANA M.; LEITE, SUZANE K. M.; TERRA, AKYRA; WUTKE, ROGER DE M. M.; REZENDE, ANTONIO C. S.; REZENDE, JOSE R. S.; et al. Reproductive performance of yearling Bos indicus heifers submitted to programs for induction of ovulation prior to a timed-artificial insemination protocol. Theriogenology, v. 241, p. 12-pg., 2025-07-15. (18/03798-7)

CONSENTINI, CARLOS E. C.; ABADIA, TATTIANY; GALINDEZ, JUAN P. A.; LOPES, ANA L. M.; FERRO, PEDRO P. C.; PAZINI, YASMIM E.; FARIA, NATALIA, V; MACHADO, FERNANDO; CAPELLA, TUANNE; DOS SANTOS, TIAGO N.; et al. Fertility programs for lactating dairy cows: A novel presynch plus timed artificial insemination program (Double E-Synch) produces similar ovarian dynamics, synchronization, and fertility as Double-Ovsynch. JOURNAL OF DAIRY SCIENCE, v. 108, n. 4, p. 13-pg., 2025-04-15. (18/03798-7)

ALVES, RODRIGO L. O. R.; SILVA, LUCAS O. E; SILVA, MATEUS A. DA; CONSENTINI, CARLOS E. C.; GONCALVES, JOSE R. S.; MELO, LEONARDO DE F. E; RODRIGUES, MARCELO B.; SARTORI, ROBERTO. Timed-artificial insemination protocols for Bos indicus beef heifers: Evaluation of protocol length and variations in prostaglandin F2α treatments. Theriogenology, v. 223, p. 9-pg., 2024-05-08. (18/14723-8, 18/03798-7)

SILVA, LUCAS O. E; MOTTA, JESSICA C. L.; OLIVA, ABRAHAM L.; MADUREIRA, GUILHERME; ALVES, RODRIGO L. O. R.; FOLCHINI, NATALIA P.; DA SILVA, MATEUS A.; DA SILVA, TAYNARA J. B.; CONSENTINI, CARLOS E. C.; WILTBANK, MILO C.; et al. Influence of GnRH analog and dose on LH release and ovulatory response in Bos indicus heifers and cows on day seven of the estrous cycle. Theriogenology, v. 214, p. 9-pg., 2023-11-03. (18/03798-7)

SARTORI, R.; FOLCHINI, N. P.; DEMETRIO, D.; BARUSELLI, P. S.. Applied use of embryo technologies in dairy cattle.. JOURNAL OF DAIRY SCIENCE, v. 105, p. 1-pg., 2022-01-01. (18/03798-7)

PRATA, ALEXANDRE B.; MADUREIRA, GUILHERME; ROBL, ADELINO J.; RIBEIRO, HEULLER S.; SAGAE, MILTON; ELIAS, V, MANOEL C.; PIMENTA, CESAR; BARRIOS, JHONNY; HARTMMAN, DIEGO; SCHNEIDER, ALTHUIR A.; et al. Progesterone-based timed AI protocols for Bos indicus cattle III: Comparison of protocol lengths. Theriogenology, v. 152, p. 29-35, AUG 2020. (18/03798-7)

CARDOSO CONSENTINI, CARLOS EDUARDO; WILTBANK, MILO CHARLES; SARTORI, ROBERTO. Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs. ANIMALS, v. 11, n. 2, FEB 2021. (17/15904-3, 18/03798-7)

DRUM, JESSICA N.; MADUREIRA, GUILHERME; ROSA, CAMILA O.; SENEDA, MARCELO M.; WILTBANK, MILO C.; SARTORI, ROBERTO; ORTEGA, M. SOFIA. Male Embryos Produced in vitro Deviate From Their in vivo Counterparts in Placental Gene Expression on Day 32 of Pregnancy. FRONTIERS IN ANIMAL SCIENCE, v. 3, p. 12-pg., 2022-02-16. (18/03798-7)

SILVA, LUCAS OLIVEIRA E; FOLCHINI, NATALIA P.; ALVES, RODRIGO L. O. R.; MADUREIRA, GUILHERME; CONSENTINI, CARLOS E. C.; MOTTA, JESSICA C. L.; WILTBANK, MILO C.; SARTORI, ROBERTO. Effect of progesterone from corpus luteum, intravaginal implant, or both on luteinizing hormone release, ovulatory response, and subsequent luteal development after gonadotropin-releasing hormone treatment in cows. JOURNAL OF DAIRY SCIENCE, v. 106, n. 6, p. 16-pg., 2023-05-26. (18/03798-7)

CONSENTINI, CARLOS E. C.; ALVES, RODRIGO L. O. R.; SILVA, MATEUS A.; GALINDEZ, JUAN P. A.; MADUREIRA, GUILHERME; LIMA, LAISSE G.; GONCALVES, JOSE R. S.; WILTBANK, MILO C.; SARTORI, ROBERTO. What are the factors associated with pregnancy loss after timed-artificial insemination in Bos indicus cattle?. Theriogenology, v. 196, p. 6-pg., 2023-01-15. (18/03798-7)

MOTTA, JESSICA C. L.; MADUREIRA, GUILHERME; SILVA, LUCAS O.; ALVES, RODRIGO L. O. R.; SILVESTRI, MAYARA; DRUM, JESSICA N.; CONSENTINI, CARLOS E. C.; PRATA, ALEXANDRE B.; POHLER, KY G.; WILTBANK, MILO C.; et al. Interactions of circulating estradiol and progesterone on changes in endometrial area and pituitary responsiveness to GnRH. BIOLOGY OF REPRODUCTION, v. 103, n. 3, p. 643-653, SEP 2020. (18/03798-7, 17/16701-9, 18/09266-7)

CONSENTINI, CARLOS E. C.; CARNEIRO, TIAGO O.; NERI, HUMBERTO; BATISTA, EMILIANA O. S.; SILVA, LUCAS O. E; SOUZA, ALEXANDRE H.; SARTORI, ROBERTO. Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows. JDS COMMUNICATIONS, v. 3, n. 3, p. 6-pg., 2022-05-01. (17/15904-3, 18/03798-7)

SARTORI, ROBERTO; ALVES, RODRIGO LEMOS OLIVIERI RODRIGUES; LOPES, ANA LUIZA MULLER. Induction of puberty vs. induction of ovulation using steroid hormones in beef heifers: a comprehensive review. ANIMAL REPRODUCTION, v. 21, n. 3, p. 16-pg., 2024-01-01. (21/09904-6, 18/03798-7)

ALVES, RODRIGO L. O. R.; SILVA, LUCAS O. E.; CONSENTINI, CARLOS E. C.; SANTOS, OSEIAS P.; NARVAEZ, ANDRES A. C.; DA CUNHA, LEANDRO D.; BRAZ, RAFAEL A.; SILVA, MARIA E. R. E.; BRUENER, RODRIGO; MELO, LEONARDO F.; et al. Reproductive outcomes of prepubertal Bos indicus beef heifers raised in a feedlot system submitted or not to ovulation induction strategies prior to a timed-artificial insemination treatment regimen. Theriogenology, v. 243, p. 12-pg., 2025-05-02. (21/09904-6, 18/03798-7)

CONSENTINI, CARLOS E. C.; MELO, LEONARDO F.; MOTTA, JESSICA C. LEMOS; OLIVEIRA E SILVA, LUCAS; FOLCHINI, NATALIA PICOLI; ALVES, RODRIGO L. O. R.; MADUREIRA, GUILHERME; CARNEIRO, TIAGO OLIVEIRA; ABADIA, TATTIANY; MOROZ, LUIS; et al. Reproductive outcomes of lactating dairy cows submitted to first timed artificial insemination protocols with different strategies to induce final ovulation. JOURNAL OF DAIRY SCIENCE, v. 108, n. 1, p. 12-pg., 2024-12-24. (17/15904-3, 18/03798-7)

CONSENTINI, CARLOS E. C.; MELO, LEONARDO F.; ABADIA, TATTIANY; GONZALES, BRUNO; MOTTA, JESSICA C. L.; ALVES, RODRIGO L. O. R.; SILVA, LUCAS O. E; WILTBANK, MILO C.; SARTORI, ROBERTO. Comparison of gonadotropin-releasing hormone and estradiol benzoate plus gonadotropin-releasing hormone to initiate a progesterone-based timed artificial insemination resynchronization protocol in lactating dairy cows. JOURNAL OF DAIRY SCIENCE, v. 107, n. 7, p. 10-pg., 2024-06-26. (17/15904-3, 18/03798-7)